BIOCHEMISTRYANDMOLECULARBIOLOGYThe double helix of DNA modelDNA molecular are paired in anantiparallel manner- that is , the5'end of one strand is paired withthe 3'-end of the other strand. TheMinorgroovebackboneofdoublehelixisdeoxyribose-phosphate,thetwo3.41chains are right hand coiled arounda common axis.The helix diameter isMajorgroove2nm, the two strands in the helixcreates a major(wide) groove and aminor(narrow) groove.Department of Biochemistry and Molecular BiolagyMedicingSchool2.0nm
Department of Biochemistry and Molecular Biology, Medicine School BIOCHEMISTRY AND MOLECULAR BIOLOGY ◆ DNA molecular are paired in an antiparallel manner- that is , the 5’end of one strand is paired with the 3’-end of the other strand. The backbone of double helix is deoxyribose-phosphate, the two chains are right hand coiled around a common axis.The helix diameter is 2nm，the two strands in the helix creates a major(wide) groove and a minor(narrow) groove. Major groove Minor groove The double helix of DNA model
BIOCHEMISTRYANDMOLECULARBIOLOGYThe double helix of DNA modelThe. base is perpendicularwith inside of double helixaxis, pairing with hydrogenMinorgroovetooppositebase(A=T;P3.4nmG=C)GThe distance of the plane ofMajorgroovevicinage base is 0.34nm,screw pitch is 3.4nm, 10bases are one round.Department of Biochemistry and MolecularBiologyMedicineSchool2.0nm
Department of Biochemistry and Molecular Biology, Medicine School BIOCHEMISTRY AND MOLECULAR BIOLOGY ◆The base is perpendicular with inside of double helix axis, pairing with hydrogen to opposite base(A=T; GC） . ◆The distance of the plane of vicinage base is 0.34nm， screw pitch is 3.4nm，10 bases are one round. Minor groove Major groove The double helix of DNA model
BIOCHEMISTRYANDMOLECULARBIOLOGYBase pairingCGTAHydrogenbondHydrogen bondCHttt1000000oHDepartment of Biochemistry and Molecular Biology,Medicine School
Department of Biochemistry and Molecular Biology, Medicine School BIOCHEMISTRY AND MOLECULAR BIOLOGY Base pairing A T C G Hydrogen bond Hydrogen bond
BIOCHEMISTRYANDMOLECULARBIOLOGYThe double helix of DNA modelHydrogen bond make thehorizontal double helixMinorgroovestable, the base stack force3.4nmmaintain the verticalMajorgroovedouble helix stable.2.0nmDepartment of Biochemistry and Molecular Biology,Medicine School
Department of Biochemistry and Molecular Biology, Medicine School BIOCHEMISTRY AND MOLECULAR BIOLOGY Minor groove Major groove Hydrogen bond make the horizontal double helix stable, the base stack force maintain the vertical double helix stable. The double helix of DNA model
BIOCHEMISTRYANDMOLECULARBIOLOGYThe essence of double helixmodelComponent :The two DNA strands antiparalleland pairing with base by hydrogen bondDirection: right hand screwStability force: horizontal direction-base pairingwith hydrogen bond; vertical direction-basestack forceReference: A screw one round-10bp, 0.34nmDepartment of Biochemistry and Molecular Biology,Medicine School
Department of Biochemistry and Molecular Biology, Medicine School BIOCHEMISTRY AND MOLECULAR BIOLOGY The essence of double helix model Component ：The two DNA strands antiparallel and pairing with base by hydrogen bond Direction：right hand screw Stability force：horizontal direction-base pairing with hydrogen bond；vertical direction-base stack force Reference：A screw one round-10bp，0.34nm