Virus isolation-cell cultures Cell Cultures are most widely used for virus isolation, there are 3 types of cell cultures 1. Primary cells- Monkey Kidney 2. Semi-continuous cells- Human embryonic kidney and skin fibroblasts 3. Continuous cells -Hela, Vero, Hep2, Llc-mK2, mdck Primary cell culture are widely acknowledged as the best cell culture systems available since they support the widest range of viruses. However they are very expensive and it is often difficult to obtain a reliable suppe Continuous cells are the most easy to handle but the range of viruses supported is often limited
Virus Isolation-cell cultures Cell Cultures are most widely used for virus isolation, there are 3 types of cell cultures: 1. Primary cells - Monkey Kidney 2. Semi-continuous cells - Human embryonic kidney and skin fibroblasts 3. Continuous cells - HeLa, Vero, Hep2, LLC-MK2, MDCK Primary cell culture are widely acknowledged as the best cell culture systems available since they support the widest range of viruses. However, they are very expensive and it is often difficult to obtain a reliable supply. Continuous cells are the most easy to handle but the range of viruses supported is often limited
Cultured cells Primary a Heterogeneous-many cell types 口C| osest to anima 口 Technica| hassle a Diploid cell strain a Relatively homogeneous- fewer cell types 口 Further from animal a Technically less hassle Continuous cell line 口| morta 口 Most homogeneous a Genetically weird -furthest from animal 口Hass| e free a Suspension or monolayer
Cultured cells Primary Heterogeneous – many cell types Closest to animal Technical hassle Diploid cell strain Relatively homogeneous – fewer cell types Further from animal Technically less hassle Continuous cell line Immortal Most homogeneous Genetically weird – furthest from animal Hassle free Suspension or monolayer
Laminar flow Hoods Decontaminated air a virologist's facility HEPA filter Glass shield Laminar vertical flow hoods HEPA filter 口 Contains hepa filter ■ Removes9997%of particles of 0. 3uM or higher Blower -O Figure 5-3: Vertical laminar flow hood
Laminar Flow Hoods Virologist’s facility Laminar vertical flow hoods Contains HEPA filter Removes 99.97% of particles of 0.3μM or higher Figure 5-3: Vertical laminar flow hood
Primary cell culture t enzymes time
Primary cell culture + enzymes time
Subculture enzymes time
Subculture enzymes time