ndirect examination 1. Cell culture cytopathic effect (CPE) haemadsorption Immunofluorescence 2. Eggs pocks on Cam haemagglutination inclusion bodies 3. Animals isease or deat th
Indirect Examination 1. Cell Culture cytopathic effect (CPE) haemabsorption immunofluorescence 2. Eggs pocks on CAM haemagglutination inclusion bodies 3. Animals disease or death
Virus isolation -animal inoculation Choice of animal and inoculation pathways Mouse, rat, rabbit, monkey, and so on
Choice of animal and inoculation pathways Mouse, rat, rabbit, monkey, and so on. Virus Isolation-animal inoculation
Virus isolation-embryos inoculation Chorioallantoic membrane inoculation (chorionic epithelium) Amniotic cavity Shell Air sac kolA Amniotic inoculation (amniotic epithelium) Yolk sac inoculation yolk sac lining) Yolk sac Allantoic inoculation (atlantic epithelium) cav Chorioallantoic membrane Davis, Duylbecco, Eisen, Ginsberg "Microbiology" 4th ed, J.B. Lippincott 1990, Fig 48-1
Davis, Duylbecco, Eisen, Ginsberg “Microbiology” 4th ed, J.B. Lippincott 1990, Fig. 48-1 Virus Isolation-embryos inoculation
d 3 d 5 d7 d10 d12 d16
d3 d5 d7 d10 d12 d16
9-11 days embryos taken out after virus infection Ⅴ irus propagation and vaccine production in enterprise industry
9-11 days embryos taken out after virus infection Virus propagation and vaccine production in enterprise industry