4Instruments>Test tubes>Pipettes, disposable tips, rack>Water bath>Colorimeter
4 Instruments Test tubes Pipettes, disposable tips, rack Water bath Colorimeter
5Procedure5.1 Label three clean, dry test tubes as Blank (B), Standard (S), and Test (T)5.2 Pipette as follows:BlankTestStandard1 mL1 mL1 mLGOD-PODReagent10 uLDistilledwater10 μuLGlucose standard一10 μLSample-5.3 Mix well and incubate at 37°C for 10 min. Or, at 25°C for 30 min5.4 Measure the ODs4o of the standard and test sample against the blank
5 Procedure Blank Standard Test GOD-POD Reagent 1 mL 1 mL 1 mL Distilled water 10 µL – – Glucose standard – 10 µL – Sample – – 10 µL 5.1 Label three clean, dry test tubes as Blank (B), Standard (S), and Test (T). 5.2 Pipette as follows: 5.3 Mix well and incubate at 37℃ for 10 min. Or, at 25℃ for 30 min. 5.4 Measure the OD540 of the standard and test sample against the blank