page 17 Chapter 2: Getting Started-Basic Imaging Functions Chapter Overview Basic Imaging Functions Automatic Image Capture(AlC)Software-Optional .AlphaView System Quick Guide Contrast Adjustments ·Imaging Tools FlorChem HD2 and FC2 User Guide
page 17 FluorChem HD2 and FC2 User Guide Chapter 2: Getting Started—Basic Imaging Functions Chapter Overview • Basic Imaging Functions • Automatic Image Capture (AIC) Software–Optional • AlphaView System Quick Guide • Contrast Adjustments • Imaging Tools
page 8 Chapter 2:Getting Started-Basic maging Functions Basic Imaging Functions Power up the system computer and click on the AlphaView icon on the desktop to open AlphaView soft- ware. Alpha viow @ es0n:3200 GaprgrfSge0200ANRgEsReand Figure 2-:AlphaView Screen-Displaying the Image Area and Display Controls AlphaView software has four main control areas: Acquire for image acquisition Contrast Adjustments for image adjustment Enhancement Tools for image enhancement Analysis Tools for image analysis Acquiring an Image Using the Navigator ingh vde theuerhougmnuepiinh example procedure,the"Ethidium Bromide"application is demonstrated. FluorChem HD2and FC2User Guide
page 18 Chapter 2: Getting Started—Basic Imaging Functions FluorChem HD2 and FC2 User Guide Basic Imaging Functions Power up the system computer and click on the AlphaView icon on the desktop to open AlphaView software. Figure 2-1: AlphaView Screen—Displaying the Image Area and Display Controls AlphaView software has four main control areas: • Acquire for image acquisition • Contrast Adjustments for image adjustment • Enhancement Tools for image enhancement • Analysis Tools for image analysis Acquiring an Image Using the Navigator Clicking the Navigator toolbar icon will guide the user through commonly used applications; in this example procedure, the “Ethidium Bromide” application is demonstrated
Basic Imaging Functions page19 OYet.I reed atsistance wth image acquition ccw☐以e, Figure 2-2:AlphaNavigator-Selecting the Yes Option Selecting the"Yes.option displays the following dialog box. AlphaNavigator Select samplo type ⊙d Chemluminetcence Bio Colory Plate 月 Figure 2-3:Selecting the Sample Type NOTE:The supported applications depend on the product Selecting Gel displays the following screen: FluorChem HD2 and FC2 User Guide
Basic Imaging Functions page 19 FluorChem HD2 and FC2 User Guide Figure 2-2: AlphaNavigator—Selecting the Yes Option Selecting the “Yes, …” option displays the following dialog box. Figure 2-3: Selecting the Sample Type NOTE: The supported applications depend on the product. Selecting Gel displays the following screen:
Seledt gel type Nuceic Acid ●Protein Selecting Nucleic Acid displays the following screen: Select stoin Figure 2-4:Selecting the Ethidium Bromide Stain Option Choosing Ethidium Bromide will select the proper light source and filter needed.The acquisition screen in the preview mode is displayed with the Auto Expose option selected. FluorChem HD2 and FC2User Guid
page 20 Chapter 2: Getting Started—Basic Imaging Functions FluorChem HD2 and FC2 User Guide Selecting Nucleic Acid displays the following screen: Figure 2-4: Selecting the Ethidium Bromide Stain Option Choosing Ethidium Bromide will select the proper light source and filter needed. The acquisition screen in the preview mode is displayed with the Auto Expose option selected
Basic Imaging Functions page 21 and Prer Figure 2-5:Previewing Your Image AlphaNavigator displays navigation notes.Click on the Advanced button to access more acquisition controls or click Next to acquire.If the Advanced button is selected: FluorChem HD2 and FC2 User Guide
Basic Imaging Functions page 21 FluorChem HD2 and FC2 User Guide Figure 2-5: Previewing Your Image AlphaNavigator displays navigation notes. Click on the Advanced button to access more acquisition controls, or click Next to acquire. If the Advanced button is selected: