Readins OIn all cases, look first at the control tubes and proceed only if they show satisfactory results. There should be no appreciable sedimentation of the bacteria 9 Pick up the individual tubes of each row of the patients specimens and look irst at the supernatant D When a complete agglutination occurs, practically all the bacteria are removed from the supernatant which appears absolutely clear o When the reaction is negative the suspension should look as turbid as the antigen control is O The agglutination of O antigen appears as a matt or"carpet"at the bottom D Agglutination of H antigens appears loose, wooly or cottony. The highest dilution of serum that produces a positive agglutination is taken as titre The titres for all the antigens are noted The in-between reactions can be categorized into+,++,+++
11 In all cases, look first at the control tubes and proceed only if they show satisfactory results. There should be no appreciable sedimentation of the bacteria. Pick up the individual tubes of each row of the patient’s specimens and look first at the supernatant. When a complete agglutination occurs, practically all the bacteria are removed from the supernatant which appears absolutely clear. When the reaction is negative the suspension should look as turbid as the antigen control is. The agglutination of O antigen appears as a “matt” or “carpet” at the bottom. Agglutination of H antigens appears loose, wooly or cottony. The highest dilution of serum that produces a positive agglutination is taken as titre. The titres for all the antigens are noted. The in-between reactions can be categorized into +, ++, +++. Reading
Broth inoculation 1. To inoculate a broth culture, hold a labeled broth tube in your left hand and your loop in your right hand using the thumb and first two fingers 2. Flame to sterilize the loop 3. Remove the cap from the broth tube with the little finger of the right hand curling aga ainst your palm 4. Flame the opening of the broth tube ouch the loop to the desired colony on the plate and then stir the loop around in the broth 6. Reflame the opening of the tube, return the cap, and place the tube in a test tube rack 7. Flame sterilize the loop 8. Incubate inoculated broth at the appropriate atmospheric conditions, temperature(37 C), and time(16-24hs) 9. Examine broth for any signs of growth including, turbidity with or without gas bubbles, hemolysis (in blood cultures), pellicle formation and precipitate on the bottom of the tube or bottle
12 1. To inoculate a broth culture, hold a labeled broth tube in your left hand and your loop in your right hand using the thumb and first two fingers 2. Flame to sterilize the loop 3. Remove the cap from the broth tube with the little finger of the right hand curling against your palm 4. Flame the opening of the broth tube 5. Touch the loop to the desired colony on the plate and then stir the loop around in the broth 6. Reflame the opening of the tube, return the cap, and place the tube in a test tube rack 7. Flame sterilize the loop. 8. Incubate inoculated broth at the appropriate atmospheric conditions, temperature(37 ℃ ), and time(16-24hs). 9. Examine broth for any signs of growth including, turbidity with or without gas bubbles, hemolysis (in blood cultures), pellicle formation and precipitate on the bottom of the tube or bottle. Broth Inoculation