RecombinantvaccinesEngerixB,hepatitisBvaccineproduced inyeast,developed byGlaxoSmithKline.二十面体核衣小表西要白loosahedral core (C)Small surface protein (S)中泰西蛋白Medumsuceprotei(S-PreS2)大表面蛋白cepro(SPle52+PreS1)20mcg/mLDNANDC58160-860-01HEPATITISBVACCINRECOMBINANT)ENGERIX-BOPolymerase (P)聚合TAdultDoseHepatitisBvirusC2002JamesA.Perkins9-11
9-11 Copyright © The McGraw-Hill Companies, Inc. Permission required to reproduce or display Engerix Engerix B, hepatitis B hepatitis B vaccine produced in yeast, developed by vaccine produced in yeast, developed by GlaxoSmithKline. GlaxoSmithKline. Recombinant vaccines Recombinant vaccines
E.collARSensequences20mcg/mLNDC58160860-01HEPATITISBVACCINRECOMBINANTENGERIX-BSelectionmarker1AdtiltDoseHBsAgexpression cassetteDane particlesKE(Φ 42 nm)9-12
9-12 Copyright © The McGraw-Hill Companies, Inc. Permission required to reproduce or display Dane particles ( 42 nm)
VaccinesagainstcancerGardasil,a subunitvaccine againsthumanpapillomavirus (HPV,人乳头瘤病毒）thatcause~70%ofcervicalcancers.DevelopedbyMerckandwasapprovedbyFDAin2005Humanpapillomavirus(HPV)SexuallytransmittedHPvinfectionsare commonand oftenasymptomatic,untreatedcasesinwomenarethemaincauseofcervicalcanceAsexuallyMorethan15havebeenVtransmitted100typesofidentifiedasHPVhavevirusthatputtingwomenbeenfoundathighriskforcausescancersofarcervicalcancerCervical cancerVirus in cervixHPV90percentof0.8percentof2enterscellscaseshealcasesdevelopreplicatesortHsthroughmicrowithintwoyearscancerabrasions6.11.161EUDASILSeveral10-30书yearsweekslaterlaterInfects cellsInfectionspreadsHPV invades deeperlayeroftissuesandturnscancerousAFPSource:NobeFDA9-13
9-13 Copyright © The McGraw-Hill Companies, Inc. Permission required to reproduce or display Vaccines against cancer Vaccines against cancer Gardasil Gardasil, a subunit vaccine against , a subunit vaccine against human papillomavirus papillomavirus (HPV,人乳 头瘤病毒) that cause ~ 70% of cervical cancers. Developed by Merck that cause ~ 70% of cervical cancers. Developed by Merck and was approved by FDA in 2005. and was approved by FDA in 2005
Humanpapillomavirus（HPV，人乳头瘤病毒）DiseaseHPVtype2.7.22Commonwarts1,2,4, 63Plantarwarts3.10.8Flatwarts6, 11,42, 44 and others[13]Anogenital warts6,16,18,31,53,5[14]Analdysplasia(lesions).Highest risk:131 16. 18, 31.45. Other high-risk:[13115] 33, 35, 39, 51, 52, 56, 58, 59Genital cancersProbably high-risk/15] 26, 53, 66, 68, 73, 82Epidermodysplasiaverruciformismorethan15types13.32Focalepithelialhyperplasia(oral)Oralpapillomas6,7.11.16,3216Oropharyngeal cancer60Verrucous cyst6.11Laryngealpapillomatosis9-14
9-14 Copyright © The McGraw-Hill Companies, Inc. Permission required to reproduce or display Human papillomavirus (HPV, 人乳头瘤病毒)
Edible vaccinesGenefromahumanVaccines arepathogen is insertedproduced ininto avectorplantsVectorintroducedintoplant cellsEating bananaLeaf segments sprout intotriggersimmunewholeplants carryingresponsetopathogengenefrom human pathogenFig22-29-15
9-15 Copyright © The McGraw-Hill Companies, Inc. Permission required to reproduce or display Fig 22-2 Edible vaccines Edible vaccines Vaccines are Vaccines are produced in produced in plants