Summary of Qnion 4.Light's Requirement of Onion >14h Long Day UNITED STATES 11.5h-14h Day Neutral <11.5h Short Day 。6号 4 Antaretiea
Long Day Day Neutral Short Day ＞ 14 h 11.5h~14 h ＜ 11.5h 4. Light's Requirement of Onion Summary of Onion
Onion tissue culture Techniques 1.Culture of meristem >Much research on the culture of onion stem tip.MS, attached growth regulators such as 6-BA and NAA, was used as the medium
Onion tissue culture Techniques 1. Culture of meristem ➢Much research on the culture of onion stem tip. MS , attached growth regulators such as 6-BA and NAA , was used as the medium
Onion tissue culture Techniques 2.Organ culture There are many types of explants:bulbs,young leaves,flower organs,scales,etc. The onion scales plate is considered to be an effective method for producing tube seedlings. Buds and leaves are also easier to induce plant regeneration.Pike and Yoo et al.directly induced the bud from the floret by using MS medium with 5 mg/L 6BA and NAA's
2. Organ culture There are many types of explants: bulbs, young leaves, flower organs, scales, etc. The onion scales plate is considered to be an effective method for producing tube seedlings. Buds and leaves are also easier to induce plant regeneration. Pike and Yoo et al. directly induced the bud from the floret by using MS medium with 5 mg/L 6BA and NAA's . Onion tissue culture Techniques
Onion tissue culture Techniques The callus induced of unopened tender inflorescence(1.5-2.0cm) Treatment 2,4 6- No.of explants No.of callus Rate of callus D(mg/L)BA(mg/L) induction B-1 0.5 1.0 58 48 82.76% B-2 0.5 2.0 30 24 80.00% B-3 0.5 4.0 55 47 85.45% B-4 1.0 1.0 60 59 98.33% B-5 1.0 2.0 41 36 87.80% B-6 1.0 4.0 58 49 84.48% B-7 2.0 1.0 53 46 86.79% B-8 2.0 2.0 38 29 76.32% B-9 2.0 4.0 40 35 87.50% Averag 48.11 41.44 85.49%
Treatment 2,4- D(mg/L) 6- BA(mg/L) No. of explants No. of callus Rate of callus induction B- 1 0.5 1.0 58 48 82.76% B- 2 0.5 2.0 30 24 80.00% B - 3 0.5 4.0 55 47 85.45% B - 4 1.0 1.0 60 59 98.33% B - 5 1.0 2.0 41 36 87.80% B - 6 1.0 4.0 58 49 84.48% B - 7 2.0 1.0 53 46 86.79% B - 8 2.0 2.0 38 29 76.32% B - 9 2.0 4.0 40 35 87.50% Averag 48.11 41.44 85.49% The callus induced of unopened tender inflorescence (1.5-2.0cm) Onion tissue culture Techniques
Onion tissue culture Techniques a.Calli induced from explants A after 4 weeks on medium b.Calli induced from explant B after 8 weeks on B5 medium with 1.0 mg L1 2,4-D +1.0 mgL!6-BA; c c.Callus multiplication after 4 weeks on B5 medium with 0.5 mg L!2,4-D 1.0 mg L! 6-BA; d.Callus differentiate regeneracy shoot after 2 weeks on B5 medium with 1.0 mg L!TDZ: e.Bud clusters differentiated after 6 weeks on B5 medium with 1.0 mgL!TDZ; f.Multiplication and development of regeneracy shoot on B5 medium with 0.01 mg L-!TDZ; g-h.Regeneracy plantlets rooted after 4 weeks on 1/2MS medium with 0.01 mg L-I NAA; g h i.Domestication and transplantation. Fig.1 Plant regeneration from young umbel culture of onion
Fig. 1 Plant regeneration from young umbel culture of onion a. Calli induced from explants A after 4 weeks on medium ; b. Calli induced from explant B after 8 weeks on B5 medium with 1.0 mg·L-1 2,4-D + 1.0 mg·L-1 6-BA; c. Callus multiplication after 4 weeks on B5 medium with 0.5 mg·L-1 2,4-D + 1.0 mg·L-1 6-BA; d. Callus differentiate regeneracy shoot after 2 weeks on B5 medium with 1.0 mg·L-1 TDZ; e. Bud clusters differentiated after 6 weeks on B5 medium with 1.0 mg·L-1 TDZ; f. Multiplication and development of regeneracy shoot on B5 medium with 0.01 mg·L-1 TDZ; g-h. Regeneracy plantlets rooted after 4 weeks on 1/2MS medium with 0.01 mg·L-1 NAA; i. Domestication and transplantation. Onion tissue culture Techniques