上泽充通大￥ Shanghai Jiao Tong University 1896 1920 1987 2006 Lecture 12-2 Microbial Genomics Chapter 12 in BROCK BIOLOGY OF MICROORGANISMS AIJIAO TONG UNI Chen Feng School of Life Science and Technology, Shanghai Jiao Tong University
1896 1920 1987 2006 Lecture 12-2 Microbial Genomics Chen Feng School of Life Science and Technology, Shanghai Jiao Tong University Chapter 12 in BROCK BIOLOGY OF MICROORGANISMS
上帝充通大睾 Shanghai Jiao Tong University 1896 1920 1987 2006 II.Gene Function and Regulation SHANCHALIIAO TONG UNIVERSITY
1896 1920 1987 2006 II. Gene Function and Regulation
12.5 Microarrays /DNA silica chips and Transcriptome基因芯片DA芯片与转录组 Microarrays/gene chips are small solid-state supports to which genes of an organism are affixed 受 and spatially arrayed in a known pattern.基因芯片 GeneChip These genes (or gene fragments)can be hybridized Human Genome U133 Plus 2.0 using a variety of probes and then scanned and analyzed by a computer. PN52001g Transcriptome:the entire complemnet of RNA Lot#:SAMPLES Exp Date:00/00/00 For Research Use Only produced under a given set of conditions..转录组 Figure 15-16a Brock Biology of Microorganisms 11/e 2006 Pearson Prentice Hall,Inc. Chen Feng,Lecture of Microbiology
Chen Feng, Lecture of Microbiology 12.5 Microarrays /DNA silica chips and Transcriptome 基因芯片/DNA芯片与转录组 Microarrays/ gene chips are small solid-state supports to which genes of an organism are affixed and spatially arrayed in a known pattern. 基因芯片 These genes (or gene fragments) can be hybridized using a variety of probes and then scanned and analyzed by a computer. Transcriptome: the entire complemnet of RNA produced under a given set of conditions.转录组
NC UN 零 4501900000000000005AMs41 GeneChip Human Genome U133Pus2.0 P/N.520019 Lot #SAMPLES Exp Date:00/00/00 For Research Use Only (a) 2012 Pearson Education,Ine. (b) 2012 Pearson Education,Ine Chen Feng,Lecture of Microbiology
Chen Feng, Lecture of Microbiology
GeneChip Probe Array Hybridized Probe Feature Single stranded.fluorescently labeled DNA target Oligonucleotide probe 24 um Each probe feature contains millions of copies of a specific oligonucleotide probe Over 200,000 different probes complementary to genetic 1.28cm information of interest Image of Hybridized Probe Array The DNA chip contains probes designed to represent thousands or tens of thousands of genes.DNA芯片中可包含上万基因的探针
The DNA chip contains probes designed to represent thousands or tens of thousands of genes.DNA芯片中可包含上万基因的探针