上泽充通大￥ Shanghai Jiao Tong University 1896 1920 1987 2006 通 Lecture 14-1 MMM Methods in Microbial Ecology CHAPTER22 in86 BROCK BIOLOGY OF MICROORGANISMS IAO TONG Chen Feng School of Life Science and Biotechnology, Shanghai Jiao Tong University Email:cf2001@situ.edu.cn
1896 1920 1987 2006 Lecture 14-1 Methods in Microbial Ecology Chen Feng School of Life Science and Biotechnology, Shanghai Jiao Tong University Email: cf2001@sjtu.edu.cn CHAPTER 22 in BROCK BIOLOGY OF MICROORGANISMS
上泽充通大￥ Shanghai Jiao Tong University 1896 1920 1987 2006 1.Culture-dependent analysis of microbial communities HANC 1日 基于培养的微生物群落研究 TON
1896 1920 1987 2006 I. Culture-dependent analysis of microbial communities 基于培养的微生物群落研究
14.1 Enrichment and Isolation Enrichment culture:a medium and a set of incubation conditions are chosen that are selective for the desired organism and counterselective for undesired organisms.富集 培养：培养基和培养条件可使某生物选择性地生长或不生 长 Both the culture medium and the incubation conditions are critical. Microbiology.Shanghai Jiao Tong University
Microbiology, Shanghai Jiao Tong University 14.1 Enrichment and Isolation Enrichment culture: a medium and a set of incubation conditions are chosen that are selective for the desired organism and counterselective for undesired organisms. 富集 培养: 培养基和培养条件可使某生物选择性地生长或不生 长. Both the culture medium and the incubation conditions are critical
Mineral salts medium containing mannitol but +NH4+plate lacking NH4+,NO3,or organic nitrogen. -NH4+plate Incubate Soil aerobically NH4+ +NH4+plate Flash -NH4+plate 2012 Pearson Education,Inc. The isolation of Azotobacter(aerobic N2 fixing bacteria)and its relatives.固氮菌的分离
The isolation of Azotobacter (aerobic N2- fixing bacteria) and its relatives. 固氮菌的分离 Flash
Enrichment The Winogradsky Column An artificial microbial ecosystem e Serves as a long-term source of bacteria for enrichment cultures Named for Sergei Winogradsky First used in late 19th century to study soil microorganisms Microbiology.Shanghai Jiao Tong University
Microbiology, Shanghai Jiao Tong University The Winogradsky Column • An artificial microbial ecosystem • Serves as a long-term source of bacteria for enrichment cultures • Named for Sergei Winogradsky • First used in late 19th century to study soil microorganisms Enrichment