上泽充通大¥ Shanghai Jiao Tong University 1896 1920 1987 2006 Lecture 14-2 Methods in Microbial Ecology CHAPTER22.inag后 BROCK BIOLOGY OF MICROORGANISMS IAO TONG Zhao Liping,Chen Feng School of Life Science and Biotechnology, Shanghai Jiao Tong University Email:cf2001@sjtu.edu.cn
1896 1920 1987 2006 Lecture 14-2 Methods in Microbial Ecology Zhao Liping, Chen Feng School of Life Science and Biotechnology, Shanghai Jiao Tong University Email: cf2001@sjtu.edu.cn CHAPTER 22 in BROCK BIOLOGY OF MICROORGANISMS
泽充通大¥ Shanghai Jiao Tong University 1896 1920 1987 2006 I. MEASURING MICROBIAL ACTIVITIES IN NATURE 风月y 1日9G nnvv
1896 1920 1987 2006 III. MEASURING MICROBIAL ACTIVITIES IN NATURE
上游充廷大¥ Shanghai Jiao Tong University Methods for activity measurement Radioisotopes放射性同位素 Microelectrodes微电极 Stable isotope analyses稳定同位素分析 Microbiology.Shanghai Jiao Tong University
Microbiology, Shanghai Jiao Tong University Methods for activity measurement Radioisotopes放射性同位素 Microelectrodes微电极 Stable isotope analyses 稳定同位素分析
浒充通大学 上 Shanghai Jiao Tong University 14.8 Chemical Assays,Radioisotopes, Microelectrodes In many studies,direct chemical measurements are sufficient Higher sensitivity can be achieved with radioisotopes Proper killed cell controls must be used Microbiology.Shanghai Jiao Tong University
Microbiology, Shanghai Jiao Tong University 14.8 Chemical Assays, Radioisotopes, & Microelectrodes In many studies, direct chemical measurements are sufficient • Higher sensitivity can be achieved with radioisotopes • Proper killed cell controls must be used
Sulfate reduction Photosynthesis Formalin-killed control 坚 Light Microbial activity H2S measurements Killed Lactate Dark Time Time (a)Chemical assay (b)Photosynthesis measured in natural seawater with 14C02 Sulfate reduction 14C-Glucose respiration H2 present 圣 uolnJona Killed、 H2 absent Killed Time Time (c)Sulfate reduction in mud (d)Production of 14CO2 from 14C- 0measured with 35S02- glucose
Chemical assay Photosynthesis measured in natural seawater with 14CO2 Sulfate reduction in mud measured with 35SO4 2- Production of 14CO2 from 14Cglucose Microbial activity measurements