17.5 Unidirectional transposition is initiated by the recipient MAT locus Y region TTTCAGCTTTCCGCAACAGTATA Figure 17.9 HO AAAGTCGAAAGGCGTTGTCATAT endonuclease cleaves MAT just to the right of the HO endonuclease Y region, generating sticky ends with a base TTTCAGCTTTCCGCAACA GTATA overhang. AAAGTCGAAAGGCG TTGTCATAT 情華大当
Figure 17.9 HO endonuclease cleaves MAT just to the right of the Y region, generating sticky ends with a base overhang. 17.5 Unidirectional transposition is initiated by the recipient MAT locus
Recipient 17.5 Unidirectional MAT transposition is initiated Cut at Y boundary by the recipient MAT locus Pairing with donor Figure 17.10 Cassette Dono HMR or HML substitution is initiated by a double-strand break in the Degradation of MAT recipient (MAT)locus,and may involve pairing on either side of the Y region with the Synthesis of new DNA donor (HMR or HML)locus. Recipient DNA has changed mating type 清菜大兰 Donor DNA is unchanged
Figure 17.10 Cassette substitution is initiated by a double-strand break in the recipient (MAT) locus, and may involve pairing on either side of the Y region with the donor (HMR or HML) locus. 17.5 Unidirectional transposition is initiated by the recipient MAT locus