Comparison of the crystal structures of in prokaryotic and eukaryotic RNA polymerases真核 和原核生物RNA聚合酶晶体结构 的比较。 a.Structure of RNA polymerase core enzyme from T.aquaticus. 热水生菌RNA聚合酶结构的核心 酶结构。 b.Structure of RNA polymerase Ⅱfrom yeast S.cerevisiae.酿酒酵 母A聚合酶Ⅱ的结构
2025/5/15 36 Comparison of the crystal structures of in prokaryotic and eukaryotic RNA polymerases 真核 和原核生物RNA聚合酶晶体结构 的比较。 a. Structure of RNA polymerase core enzyme from T. aquaticus. 栖 热水生菌RNA聚合酶结构的核心 酶结构。 b. Structure of RNA polymerase II from yeast S. cerevisiae .酿酒酵 母RNA聚合酶II的结构
Part 2 Synthesis of RNA in prokaryotes 原核生物RNA的合成 2025/5/15 37
2025/5/15 37 Part 2 Synthesis of RNA in prokaryotes 原核生物RNA的合成
一、Basic structure of promoter启动子的基本结构: 1.Promoter(启动子)definition: Nucleotide sequence in DNA to which RNA polymerase binds to begin transcription. 指确保转录精确而有效地起始的DNA序列。 2025/5/15 38
2025/5/15 38 一、Basic structure of promoter 启动子的基本结构: 1. Promoter (启动子)definition: Nucleotide sequence in DNA to which RNA polymerase binds to begin transcription. 指确保转录精确而有效地起始的DNA序列
RNA polymerase upstream downstream DNA DNA promoter 2025/5/15 39
2025/5/15 39
Transcription unit(转录单元): A region of DNA bounded by a promoter and a terminator that is transcribed as a single unit 是一段从启动子开始至终止子结束的DNA序 列,在一个转录单元中,RNA聚合酶从转录起 点开始沿着模板前进,直到终止子为止,转录 出一条RNA链。 2025/5/15 40
2025/5/15 40 Transcription unit (转录单元): A region of DNA bounded by a promoter and a terminator that is transcribed as a single unit. 是一段从启动子开始至终止子结束的DNA序 列,在一个转录单元中, RNA聚合酶从转录起 点开始沿着模板前进,直到终止子为止,转录 出一条RNA链