Chapter 3 DNA replication Old Old T::::A T::::A- T:::A 一G::C C1:::G- T::::A -T::::A- -T:1:E T: -T::::A-
Chapter 3 DNA replication
Contents Part1 ntroduction of DNA replication概述 Part 2 DNA replication in prokaryotes原核生 物的DNA复制 Part 3 DNA replication in eukaryotes真核生 物的DNA复制 Part4 Fidelity of replication复制的忠实性 Part 5 Application of DNA replication theory DNA复制原理的应用 2025/5/15 2
2025/5/15 2 Part 1 Introduction of DNA replication 概述 Part 2 DNA replication in prokaryotes 原核生 物的DNA复制 Part 3 DNA replication in eukaryotes 真核生 物的DNA复制 Part 4 Fidelity of replication 复制的忠实性 Contents Part 5 Application of DNA replication theory DNA复制原理的应用
Key points: 。 Concepts of semiconservative replication, replication eye,replication fork,replicon, replisome,leading strand,lagging strand. DNA的半保留复制，复制眼、复制叉、复制子、 复制体、前导链、后随链的概念； Features and function of DNA polymerase l, l,l.DNA聚合酶、l、I的性质及功能； Initiation and elongation of replication复制的 起始、延伸； 2025/5/15 3
2025/5/15 3 Key points： • Concepts of semiconservative replication, replication eye, replication fork, replicon, replisome, leading strand, lagging strand. DNA的半保留复制，复制眼、复制叉、复制子、 复制体、前导链、后随链的概念； • Features and function of DNA polymerase I, II, III. DNA聚合酶I、II、III的性质及功能； • Initiation and elongation of replication复制的 起始、延伸；
·Replication of linear DNA ends线性DNA末端的 复制 ·Fidelity control of Replication复制忠实性的控 制 Principles of polymerase chain reaction and sequencing PCR技术及DNA测序技术的原理 2025/5/15 4
2025/5/15 4 • Replication of linear DNA ends 线性DNA末端的 复制 • Fidelity control of Replication复制忠实性的控 制 • Principles of polymerase chain reaction and sequencing PCR技术及DNA测序技术的原理
Part 1 Introduction of DNA replication DNA复制的概况 一、核酸生物合成的一般规则 1.In most cases,the synthesis of DNA or RNA follow Watson- Crick base-pairing rules of A with T and G with C,and copy the pre-existed DNA strand(template).绝大多数 DNA或RNA生物合成，均按照Vatson-Crick碱基互补原则， 以拷贝预先存在的DNA链（模板链）的方式进行。 2.The direction of nucleic acid synthesis:核酸链合成的 方向只有一个：5’→3’。 3.The process of synthesis of DNA or RNA was catalyzed by specific polymerase特异的聚合酶催化合成DNA或RNA。 2025/5/15 5
2025/5/15 5 一、核酸生物合成的一般规则 1. In most cases, the synthesis of DNA or RNA follow WatsonCrick base-pairing rules of A with T and G with C, and copy the pre-existed DNA strand (template). 绝大多数 DNA或RNA生物合成，均按照Watson-Crick碱基互补原则， 以拷贝预先存在的DNA链（模板链）的方式进行。 Part 1 Introduction of DNA replication DNA复制的概况 2. The direction of nucleic acid synthesis: 核酸链合成的 方向只有一个：5’→3’。 3.The process of synthesis of DNA or RNA was catalyzed by specific polymerase 特异的聚合酶催化合成DNA或RNA