General Characteristics of the staphylococci Include a major human pathogen and skin commensals Spherical cells arranged in irregular clusters 产数学 v Gram-positive Coagulase: an important indicator t of pathogenic staphylococci Lack spores and flagell May have capsules Gram staining
General Characteristics of the Staphylococci Include a major human pathogen and skin commensals Spherical cells arranged in irregular clusters Gram-positive Coagulase: an important indicator of pathogenic staphylococci Lack spores and flagella May have capsules Gram staining
Classification I: isolate character on blood agar plate eta-hemolysis caused by a-toxin Zone of hemolysis caused by B-toxin ig ☆S. aureus Sepidermidis S saprophyticus
S.aureus S.epidermidis S. saprophyticus Classification I: isolate character on blood agar plate
Classification l: coagulase coagulase positive staphylococcus coagulase negative staphylococcus, CNS S epidermidis S saprophyticus Coagulase: an important indicator of pathogenic staphylococcus
coagulase positive staphylococcus S. aureus coagulase negative staphylococcus, CNS S. epidermidis S. saprophyticus Coagulase: an important indicator of pathogenic staphylococcus Classification II: coagulase
aureus 雙 :额想 你 v Morphology culture, and biological characteristics Y Pathogenesis: virulent factors dr Iseases Diagnostic laboratory tests v Principles of controlling staphylococcal infections
S. aureus Morphology, culture, and biological characteristics Pathogenesis: virulent factors, diseases Diagnostic laboratory tests Principles of controlling staphylococcal infections
Culture characteristics -s aureus V Facultative anaerobe Y Grows in large, round, opaque golden yellow colonies; B- hemolysis on blood agar plate Optimum temperature of 37oC v Tolerate high salt: 10%0-15% NaCl, high temperatures: 600C lh:80°30min Y Withstands extremes in pH Drug resistance: mrsa
Culture Characteristics - S. aureus Facultative anaerobe Grows in large, round, opaque, golden yellow colonies; β- hemolysis on blood agar plate Optimum temperature of 37oC Tolerate high salt: 10%-15% NaCl, high temperatures: 60oC 1h; 80oC 30min Withstands extremes in pH Drug resistance: MRSA