v Vibrio cholerae Enterotoxin Vcholerae produce a heat-labile enterotoxin consisting of 5 subunits B and 1 subunit A adenyl cyclase activated subunit a cyclic AMP secretion water/ions Diarrhea occurs--as much as 20-30 343 L/d--with resulting dehydration, shock acidosis and death Mechanism similar to Heat labile toxin of EteC 10/14/2013 Vibrios helicobacter Campylobacters/ Junqi Zhang
10/14/2013 Vibrios, Helicobacter & Campylobacters/ Junqi Zhang 11 Vibrio cholerae Enterotoxin V.cholerae produce a heat-labile enterotoxin consisting of 5 subunits B and 1 subunit A Mechanism similar to Heat labile toxin of ETEC Diarrhea occurs—as much as 20–30 L/d—with resulting dehydration, shock, acidosis, and death Adenyl cyclase activated cyclic AMP secretion water/ions subunit A
2 Cell membrane Cholera toxin GMI Vibrio cholerae Enterotoxin B Activated Cell hypersecretion of membrane pe ase electrolytes and water Cell membr Dehydration ATP CAMP shock H acidos CAMP H2O HCO 二H2O death H2O H2O No HCO 10/14/2013 Vibrios helicobacter 12 Campylobacters/ Junqi Zhang
Vibrio cholerae Enterotoxin 1 2 3 4 hypersecretion of electrolytes and water Dehydration shock acidosis death 10/14/2013 Vibrios, Helicobacter & 12 Campylobacters/ Junqi Zhang
1ce water” stool 10/14/2013 Vibrios helicobacter 13 Campylobacters/ Junqi zhang
10/14/2013 Vibrios, Helicobacter & Campylobacters/ Junqi Zhang 13 “rice water” stool
Pathogenesis a Pathology HO Do not reach the bloodstream but Lumen Bloodstream remain within the intestinal tract Virulent V cholerae organisms attach to the microvilli of the brush +Cholera toxin border of epithelial cells Multiply and liberate cholera toxin №a+ Cl and perhaps mucinase and Na+ endotoxin N Infective dose vary dependent on host gastric acidity umen Bloodstream Pathogenisis of Cholera toxin 10/14/2013 Vibrios helicobacter Campylobacters/ Junqi Zhang
10/14/2013 Vibrios, Helicobacter & Campylobacters/ Junqi Zhang 14 Pathogenisis of Cholera toxin Do not reach the bloodstream but remain within the intestinal tract Virulent V.cholerae organisms attach to the microvilli of the brush border of epithelial cells Multiply and liberate cholera toxin and perhaps mucinases and endotoxin Infective dose vary dependent on host gastric acidity Pathogenesis & Pathology