classical biotype es El Tor Serotype o1 gawa Serosubgroups Inaba Hikojima Vcholerae Phage types GenusⅤ brio Non-O1-other serovars Non-cholerae vibrios Other species V parahaemolyticus. food-associated diarrheal disease V. vulnificus: wound infection; septicemia Valginolyticus. otitis externa; wound infection 10/14/2013 Vibrios helicobacter Campylobacters/ Junqi Zhang
10/14/2013 Vibrios, Helicobacter & Campylobacters/ Junqi Zhang 6 Genus Vibrio V.cholerae Other species Serotype O1 Non-O1 -other serovars biotypes Serosubgroups Phage types E1 Tor classical Ogawa Inaba Hikojima V. parahaemolyticus: food-associated diarrheal disease V. vulnificus: wound infection; septicemia V.alginolyticus: otitis externa; wound infection Non-agglutinating vibrios Non-cholerae vibrios
Antigenic Structure Biologic Classification 口 Antigenic Structure Qa single heat-labile flagellar H antigen 9O lipopolysaccharides(confer serologic specificity a Biologic Classification(O1) classic biotypes ◆ El Tor biotypes produces a hemolysin resistant to polymyxin B 10/14/2013 Vibrios helicobacter Campylobacters/ Junqi Zhang
10/14/2013 Vibrios, Helicobacter & Campylobacters/ Junqi Zhang 7 Antigenic Structure & Biologic Classification a single heat-labile flagellar H antigen O lipopolysaccharides (confer serologic specificity ) classic biotypes El Tor biotypes produces a hemolysin resistant to polymyxin B Antigenic Structure Biologic Classification(O1)
Morphology Identification v Gram-negative v Comma shape or curved rod &rG- v actively motile by means of a polar flagellum 10/14/2013 Vibrios helicobacter Campylobacters/ Junqi Zhang
10/14/2013 Vibrios, Helicobacter & Campylobacters/ Junqi Zhang 8 Gram-negative Comma shape or curved rod actively motile by means of a polar flagellum Morphology & Identification
Growth Characteristics ferments sucrose and mannose but not arabinose o Halophilic( requiring the presence of Nacl to grow Culture convex, smooth, round colonies that are opaque and granular in transmitted light xV.cholerae grows well one(TCBs) agar a vibrios grow at a very high pH(.5-9.5) and are rapidly killed by acid 10/14/2013 Vibrios helicobacter Campylobacters/ Junqi Zhang
10/14/2013 Vibrios, Helicobacter & Campylobacters/ Junqi Zhang 9 Growth Characteristics ferments sucrose and mannose but not arabinose Halophilic( requiring the presence of NaCl to grow ) Culture convex, smooth, round colonies that are opaque and granular in transmitted light V.cholerae grows well one (TCBS) agar vibrios grow at a very high pH (8.5–9.5) and are rapidly killed by acid
control (TCBS)agar pH(8.5-9.5 10/14/2013 Vibrios helicobacter Campylobacters/ Junqi Zhang
10/14/2013 Vibrios, Helicobacter & Campylobacters/ Junqi Zhang 10 (TCBS) agar pH (8.5–9.5) Negative control