Proteomics and the ProteomeCentralDogmainmacroscopioDNAGenomeTranscriptionTranscriptomeRNATranslationProteomeProteinMetabolomeFunctionJuangRH(2006)ProteomicsGHUaLOCYLUHEIIeLI
Laboratory of plant molecular cytogenetics
武汉大学GenomeDNATranscriptionalcontrolFigure1.OverviewofthetransferofinformationfromthesequenceinthegenestothefunctioningRNAeditingcontrolNUCLEUSCYTOSOLPre-mRHAnaatuemRAproteinsofthecell (thecentral dogma)withthepossible control mechanismsindicated.AgeneRNA(DNAred)istranscribed(step1)topre-mRNAmRNAdegradationproccessingcontrolthatmaybeedited(step2)andthenprocessedcontrol(step3)toonemRNAorbyaltemativesplicingtoTranscriptomeFRNAMENAEFRNAseveral foms of mRNAs (blue)The mRNAs0RNA transportaretransported(step4)outofthenucleustothecontrolcytosol.In the cytosol,the mRNA may bedegraded(step5),ortranslated(step 6)intoprotein (green).Protein activity is controlledmRNA(step7).Proteinsmaybesynthesizedasinactivetranslationaforms that are later reversiblyorirreversiblycontrolactivatedor,altematively,theymaybesynthePhysiologiceffectsizedasactiveproteinsthatarelaterinactivatedProteins are the ultimate operating moleculesacies.motProteinproducingthephysiologiceffect(step8)invirtuallyactivitProteomecontroprotaneverymechanisminthe cell.Reprintedwithpermission fromHonore B & Ostergaard MIraciverroteliTranscriptomics andproteomics:integration?Nature Encyclopedia of the Human Genome,Vol.5,579-584(2003)NaturePublishingGroupLaboratory of plant molecular cytogenetics
Laboratory of plant molecular cytogenetics Transcriptional control RNA editing control RNA proccessing control mRNA degradation control RNA transport control mRNA translationa l control Protein activity control Physiologic effect
与基因组的关系Genome sequenceTranscriptionalcontrolGENOMICSmRNATranslational controlPrimaryproteinproductsPROTEOMICSPost-translationalcontrolFunctionalproteinproductsDetermination of geneeJainpharmaBiotechLaboratoryofplant molecular cytogenetics
Laboratory of plant molecular cytogenetics 与基因组的关系
与功能基因组的关系FUNCTIONALGENOMICSPROTEOMICSDNA isolation& cloningProteinfractionationSequencing2-DELECTROPHORESISMutationGeneProteinPost-identificationanalysisexpressiontranslationalmodificationHigh-throughput&RoboticseJainPharmaBiotechLaboratory of plant molecular cytogenetics
Laboratory of plant molecular cytogenetics 与功能基因组的关系
武汉大学Wuhan University与传统蛋白质研究的区别个体→→ 整体分析静态性质→→比较动态变化孤立个体→→ 相互作用结构→→ 功能小规模、非连续→→高通量、自动化Laboratory of plant molecular cytogenetics
Laboratory of plant molecular cytogenetics 与传统蛋白质研究的区别 n 个体→→ 整体 n 分析静态性质→→ 比较动态变化 n 孤立个体→→ 相互作用 n 结构→→ 功能 n 小规模、非连续→→ 高通量、自动化