Replica plating method-a powerful tool for screening平板复制法用于筛选 Imprint of colonies from a master plate is made onto agar plate for testing nutritional mutant Comparison of growth of colonies on master and replica plates will identify the mutants Chen Feng,Shanghai Jiao Tong Univerity
Chen Feng, Shanghai Jiao Tong Univerity Replica plating method- a powerful tool for screening 平板复制法用于筛选 Imprint of colonies from a master plate is made onto agar plate for testing nutritional mutant Comparison of growth of colonies on master and replica plates will identify the mutants
Flash Master plate;growth on complete medium Press plate onto Transfer imprint velveteen of colonies to Incubate fresh media Velveteen; sterilized ,55 Plastic Velveteen hoop with imprint Complete medium All colonies grow of all Wooden colonies block y Minimal medium Mutants do not grow 2012 Pearson Education,Inc. Using sterile velveteen cloth,an imprint of colonies from a master plate is made onto an agar plate lacking the nutrient.The colonies of the parental type will grow normally,whereas those of the mutant will not.Thus,the inability of a colony to grow on the replica plate is a signal that it is a mutant. Replica plating method for detection of nutritional mutants
Replica plating method for detection of nutritional mutants Using sterile velveteen cloth, an imprint of colonies from a master plate is made onto an agar plate lacking the nutrient. The colonies of the parental type will grow normally, whereas those of the mutant will not. Thus, the inability of a colony to grow on the replica plate is a signal that it is a mutant. Flash
Replica plating result Complete medium Minimal medium The replica plate lacked one nutrient present in the master plate. Therefore,the colonies marked with an X are auxotrophs
Replica plating result The replica plate lacked one nutrient present in the master plate. Therefore, the colonies marked with an X are auxotrophs. Complete medium Minimal medium