TONG UN Selectable Mutant Example可选择的突变株 (a) 2012 Pearson Education,Inc. Antibiotic-resistant mutants within the inhibition zone of an antibiotic assay disc.抗生素抗性突变株 Chen Feng,Shanghai Jiao Tong Univerity
Chen Feng, Shanghai Jiao Tong Univerity Selectable Mutant Example 可选择的突变株 Antibiotic-resistant mutants within the inhibition zone of an antibiotic assay disc.抗生素抗性突变株
Screening vs.Selection Screening is to observe all the cells in a population to identify mutants from parent cells筛选：从群体中观察所有细胞， 从中挑选出变异株 Selection is to grow population under certain conditions so that mutants will grow faster than parent cells (eventually they become the majority or even the only cell types in the population).选择：在特定培养条件中使变异株成为优势株 或唯一生长的菌株 Chen Feng,Shanghai Jiao Tong Univerity
Chen Feng, Shanghai Jiao Tong Univerity Screening vs. Selection Screening is to observe all the cells in a population to identify mutants from parent cells筛选：从群体中观察所有细胞， 从中挑选出变异株 Selection is to grow population under certain conditions so that mutants will grow faster than parent cells (eventually they become the majority or even the only cell types in the population). 选择：在特定培养条件中使变异株成为优势株 或唯一生长的菌株
Selection for mutants变异株的选择方法 Positive selection for mutants Drug resistance-mutants which are resistant to some antibiotic can be selected by growing on medium containing this drug药物抗性突变株可以在含该药物的培养基中生 长而被选择出来。 Negative selection which is not for the mutant but against parental type ·Penicillin selection青霉素选择法 Penicillin only kill actively growing cells Chen Feng,Shanghai Jiao Tong Univerity
Chen Feng, Shanghai Jiao Tong Univerity Selection for mutants 变异株的选择方法 Positive selection for mutants • Drug resistance-mutants which are resistant to some antibiotic can be selected by growing on medium containing this drug药物抗性突变株可以在含该药物的培养基中生 长而被选择出来。 Negative selection which is not for the mutant but against parental type • Penicillin selection青霉素选择法 • Penicillin only kill actively growing cells
上泽充通大￥ Shanghai Jiao Tong University 1896 1920 1987 2006 10.1.2 Isolation of Nutritional Auxotrophs:Replica plating method and Penicillin Selection 营养缺陷型的分离 VERS 平板复制法与青霉素负选择法
1896 1920 1987 2006 10.1.2 Isolation of Nutritional Auxotrophs: Replica plating method and Penicillin Selection 营养缺陷型的分离: 平板复制法与青霉素负选择法
Nutritional mutation营养型突变 圈Auxotroph营养缺陷型 A nutritional mutant that has a requirement for a growth factor Prototroph原养型 The wildtype parent for a nutritional mutation Chen Feng,Shanghai Jiao Tong Univerity
Chen Feng, Shanghai Jiao Tong Univerity Nutritional mutation 营养型突变 Auxotroph营养缺陷型 • A nutritional mutant that has a requirement for a growth factor Prototroph原养型 • The wildtype parent for a nutritional mutation