3.ExperimentalProcedures3.1PreparationofStandardCurvePrepare 6 clean, dry tubes and add reagents according to the following tableVolume ofVolume ofTotalCoomassieStandardProteinHO(mL)BrilliantBlueTube No.ProteinContent inAbs595Reagent(mL)Solution(mL)Tube (μg)511.0000.00025?0.2500.835?0.60.41005?40.40.6150055?0.20.82005?60.01.02500
3. Experimental Procedures 3.1 Preparation of Standard Curve Prepare 6 clean, dry tubes and add reagents according to the following table: Tube No. Volume of H₂O (mL) Volume of Standard Protein Solution (mL) Total Protein Content in Tube (μg) Coomassie Brilliant Blue Reagent (mL) Abs₅₉₅ 1 1.0 0 0 5 0.000 2 0.8 0.2 50 5 ? 3 0.6 0.4 100 5 ? 4 0.4 0.6 1500 5 ? 5 0.2 0.8 200 5 ? 6 0.0 1.0 2500 5 ?
3.Procedures> After adding all reagents, mix thoroughlyThe absorbance at 595 nm(Abss9s)canbe measured immediatelyusing a spectrophotometer.> Plot the standard curve with protein concentration as thex-axisandAbss9s asthey-axis
3. Procedures After adding all reagents, mix thoroughly. The absorbance at 595 nm (Abs₅₉₅) can be measured immediately using a spectrophotometer. Plot the standard curve with protein concentration as the x-axis and Abs₅₉₅ as the y-axis