实验失败的原因: 1漏氧气,使厌氧产氢微生物不能生长 2杂菌污染
实验失败的原因： 1 漏氧气，使厌氧产氢微生物不能生长 2 杂菌污染
经验教训 1实验中的任何一个环节都给予高度重视 不可疏漏 2.保证厌氧条件
经验教训 1.实验中的任何一个环节都给予高度重视 不可疏漏 2 .保证厌氧条件
100 1.2 -Glucose consumpt i on Soluble prote in consumpt on 0.8 OD600nm 0.6 +o月50co 40 0.4 20 0.2 02468101214161820
0 20 40 60 80 100 0 2 4 6 8 10 12 14 16 18 20 Consumption (%) 0 0.2 0.4 0.6 0.8 1 1.2 OD600 Glucose consumption Soluble protein consumption OD600nm
300 -Ethanol Ho Acetate 2000 1500 41000 500 02468101214161820 Time(h)
0 500 1000 1500 2000 2500 3000 0 2 4 6 8 10 12 14 16 18 20 Time(h) Volatile fatty acids(mg/L) Ethanol Acetate
6 2000 1500 4.5 500 3.5 02468101214161820 Time(h) Time course of growth, glucose consumption soluble protein consumption, the composition of fermentation metabolites pH, and hydrogen eⅴ olution
• Time course of growth, glucose consumption, soluble protein consumption,the composition of fermentation metabolites pH, and hydrogen evolution 3.5 4 4.5 5 5.5 6 0 2 4 6 8 10 12 14 16 18 20 Time (h) pH 0 500 1000 1500 2000 Cummulative H 2 production(ml/L-culture)