原位杂交组织化学
原位杂交组织化学
核酸分子杂交技术 固相杂交 菌落原位杂交( colony in situ hybridization) 斑点杂交法( Dot blot Southern印迹杂交( Southern blot ■ Northern印迹杂交( Northern blot) 组织原位杂交( Tissue in situ hybridization) 液相杂交
核酸分子杂交技术 固相杂交: ◼ 菌落原位杂交（colony in situ hybridization） ◼ 斑点杂交法（Dot blot） ◼ Southern印迹杂交（Southern blot） ◼ Northern印迹杂交（ Northern blot） ◼ 组织原位杂交（Tissue in situ hybridization） 液相杂交
DNA∽ DNA is digested with a restriction endonuclease 入 Restriction fragments of different sizes are separated by gel electrophoresis. Migration Paper towels Filter The DNa is denatured and transferred to a filter by passage of a salt solution through the gel Salt solution X-ray fil DNA ragments< Filter The filter is hybridized The probe bound to the with a radioactive √A filter is detected by probe, which binds to autoradiography complementary which reveals the DNA DNA sequences. fragment to which the probe hybridized
定义: 特定标记的已知序列的核酸作为探针与细胞或组织 切片中的核酸进行杂交并对标记的探针进行检测的 方法称为原位杂交
定义： 特定标记的已知序列的核酸作为探针与细胞或组织 切片中的核酸进行杂交并对标记的探针进行检测的 方法称为原位杂交
ITotal cell DNA mixture of different double-stranded molecules 95°c Denature 22 Detection of DNA Strands separate by nucleic acid Add radioactive hybridization DNA probe 65°c complementary to Renature specific cell DNA sequence Radioactive probe hybridizes to complementary sequences in cell DNA
Detection of DNA by nucleic acid hybridization