Caveolae: lipid rafts in cell surface invaginations containing caveolin raft cave。a Nae38799757
Caveolae: lipid rafts in cell surface invaginations containing caveolin Nature387(1997)571
Caveolae in endocytic traffic e Caveolae are non-clathrin coated invaginations (50-100 nm)in the plasma membrane in many cell types e Caveolae are formed by self-associating caveolin molecules(making a hairpin loop)in the membrane interacting with raft lipids e To form small signalling compartments: a number of signalling proteins are anchored, such as heterotrimeric G proteins, Src-family kinases, h-ras Be involved in endocytosis and transcytosis
Caveolae in endocytic traffic Caveolae are non-clathrin coated invaginations (50-100 nm) in the plasma membrane in many cell types. Caveolae are formed by self-associating caveolin molecules (making a hairpin loop) in the membrane interacting with raft lipids. To form small signalling compartments: a number of signalling proteins are anchored, such as hetrotrimeric G proteins, Src-family kinases, H-Ras. Be involved in endocytosis and transcytosis
Lipid composition of rafts Sphingolipid(glycosphingolipids sphingomyelin) and cholsterol sphingomyelin- and cholsterol-rich DIGs can also be isolated from cells glycosphingolipids are not absolutely required DIGS in trans-Golgi-network: rich in cholesterol and anchored by gpi protein
Lipid composition of rafts Sphingolipid (glycosphingolipids, sphingomyelin) and cholsterol sphingomyelin- and cholsterol-rich DIGs can also be isolated from cells. glycosphingolipids are not absolutely required! DIGs in trans-Golgi-network: rich in cholesterol and anchored by GPI protein!
以甘油为骨架的磷脂 即甘油分子中三个羟基有两个与高级脂肪酸形 成酯,另一个与磷酸衍生物形成酯: CHrCH-CH→CH:CHcH OH OH OH 其中R1、R2为脂肪酸碳氢链。根据X的成分不 同,可以形成不同的磷脂
以甘油为骨架的磷脂 即甘油分子中三个羟基有两个与高级脂肪酸形 成酯，另一个与磷酸衍生物形成酯： 其中R1、R2为脂肪酸碳氢链。根据X的成分不 同，可以形成不同的磷脂
以神经鞘氨醇为骨架的鞘脂类( sphingolipid) 神经鞘氨醇( sphingosine)的C-2上的氨基(NH2)与脂肪酸(R) 缩合生成神经鞘脂类( (sphingolipid,C-1上的羟基与磷酸衍 生物∞X)缩合即生成磷酸神经鞘脂类( (phosphasphingolipid) OH CH,-CH-CHOH CH,CH--CHOH CH-CH--CHOH NH: CH NH CH NH CH CO CH CO CH (CH,)t R (CHe) R (CH:). CH CH, CH 神经稍氨醇 神经鞘脂类 磷酸神经鞘脂类 若:X=磷脂胆碱(PC), 则生成神经鞘磷脂( sphingomyelin,SM 若:X=-H 则生成神经酸胺( ceramide)s
以神经鞘氨醇为骨架的鞘脂类(sphingolipid) 神经鞘氨醇(sphinogsine)的C－2上的氨基(-NH2 )与脂肪酸(R) 缩合生成神经鞘脂类(sphingolipid), C－l上的羟基与磷酸衍 生物(X)缩合即生成磷酸神经鞘脂类(phosphasphingolipid): 若: X＝磷脂胆碱(PC)， 则生成神经鞘磷脂(sphingomyelin, SM ). 若: X＝－H, 则生成神经酸胺（ceramide）