Gradients Column Foil cap 02 Algae and cyanobacteria Lake or pond water Purple nonsulfur bacteria Sulfur Mud chemolithotrophs supplemented- Patches of purple with organic sulfur or green nutrients sulfur bacteria and CaSO4 H2S Anoxic decomposition and sulfate (a) reduction 2012 Pearson Education,Inc. Winogradsky Column:a miniature anoxic ecosystem.Its has been used to isolate purple and green phototrophic bacteria, sulfate-reducing bacteria,and many other anaerobes.一个缩微 的厌氧生态系统。可用于分离紫色和绿色光合菌、硫还原细菌和其它的厌氧菌
Winogradsky Column: a miniature anoxic ecosystem. Its has been used to isolate purple and green phototrophic bacteria, sulfate-reducing bacteria, and many other anaerobes.一个缩微 的厌氧生态系统。可用于分离紫色和绿色光合菌、硫还原细菌和其它的厌氧菌
Enrichment bias Microorganisms cultured in the lab are frequently only minor components of the microbial ecosystem Reason:the nutrients available in the lab culture are typically much higher than in nature实验室培养基的 营养更易获取 Microbiology.Shanghai Jiao Tong University
Microbiology, Shanghai Jiao Tong University Microorganisms cultured in the lab are frequently only minor components of the microbial ecosystem • Reason: the nutrients available in the lab culture are typically much higher than in nature 实验室培养基的 营养更易获取 Enrichment bias
(b) 2012 Pearson Education,inc. The Winogradsky Column:remained anoxic up to the top where blooms of three different phototrophic bacteria have occurred in the mud and up into the water column
The Winogradsky Column: remained anoxic up to the top where blooms of three different phototrophic bacteria have occurred in the mud and up into the water column
14.2 Isolation Aerobes that form distinct colonies on plates are very easy to purify by culture methods. 形成单菌落的好氧菌易于纯化 Oxygen-tolerant anaerobes:plate isolations also work well if the plates are put under anoxic conditions immediately after streaking.耐氧的 厌氧菌可在划线后立即置厌氧环境培养 Microbiology.Shanghai Jiao Tong University
Microbiology, Shanghai Jiao Tong University 14.2 Isolation Aerobes that form distinct colonies on plates are very easy to purify by culture methods. 可 形成单菌落的好氧菌易于纯化 Oxygen-tolerant anaerobes: plate isolations also work well if the plates are put under anoxic conditions immediately after streaking. 耐氧的 厌氧菌可在划线后立即置厌氧环境培养
Pure cultures Pure cultures contain a single kind of microorganism Can be obtained by streak ●●● plate,agar shake,or liquid 6s●他● 66606 6ti dilution ee●6et●●ee●● (a) Colonies 2012 Pearson Education.Inc
Microbiology, Shanghai Jiao Tong University Pure cultures contain a single kind of microorganism • Can be obtained by streak plate, agar shake, or liquid dilution Pure cultures