ATPsynthaermembrane(F,)FreelypeCristandinnmocMitochondrionInnermembraneMatrixncudingHContains! Contains oxidation enzymes-RespiratoryelectrocarenCempersl-ivADPATPtIa(Except glycolytic enzymes)ATPsynthase[FF,]Drhermemanaporten Also contains DNA, ribosomes. otherMotrixCentainsenzymes,metabolic intermediates,smallPrvrCtvkacmolecules, and ionsInnermembranekeeps it separatefromncytosolic componentsPorinDNArbO-ManyotheAT.ADR.PM.C..KlanysolublemetabolicMI
Mitochondrion Matrix Contains oxidation enzymes (Except glycolytic enzymes) Also contains DNA, ribosomes, other enzymes, metabolic intermediates, small molecules, and ions Inner membrane keeps it separate from cytosolic components
Transportand shuttlesystemsHow do you move reducing equivalents from the cytosol to the matrix?Electrons donatetoETC onmatrix sideTransferelectronstomoleculethatcanbetransportedacrossmembrane(malate)Then re-oxidize molecule to extract electrons (oxaloacetate)NADH+HNADHmatrix600CoomalateCOodehydrogenasOxaloacetateMalateAspartatecytosolicmalatedehydrogenasYCOOcOCNADH+H"NADOxaloacetateMalateAspartate
Transport and shuttle systems How do you move reducing equivalents from the cytosol to the matrix? Electrons donate to ETC on matrix side Transfer electrons to molecule that can be transported across membrane (malate) Then re-oxidize molecule to extract electrons (oxaloacetate)
ATPtransporterATPMATRIXINTERMEMBRANESPACEH+P.ADP(a)(b)DohnwiSacAlrihseearvedAsymportproteinpermitsATPtranslocaseproteinimportssimultaneousmovementofPADPandexportsATPand H*
ATP transporter
Intermembranespace (p side)HElectrontransport10,+2H*H,CNADH+H+NAD+SuccinateFumarateMatrix (N side)Oxidative part of system begins with transfer of electrons torespiratorychain Electrons extracted during catabolic oxidation passed to universalacceptors like NAD+ or NADP+ (diffusible carriers) Passed sequentially through a series of membrane-bound carriers Energy used to pump protons from the matrix (N or negative side)to the intermembrane space (P or positive side) Electrons eventually passed to terminal acceptor:O,inaerobicrespiration
Electron transport Oxidative part of system begins with transfer of electrons to respiratory chain Electrons extracted during catabolic oxidation passed to universal acceptors like NAD+ or NADP+ (diffusible carriers) Passed sequentially through a series of membrane-bound carriers Energy used to pump protons from the matrix (N or negative side) to the intermembrane space (P or positive side) Electrons eventually passed to terminal acceptor: O2 in aerobic respiration
ElectrontransportsystemsSeriesofelectronacceptors Each successive acceptor in system has higher reduction potential Each has greater tendency to receive electronsMoveselectronsinonedirectionElectrondonorH'+NADH→2H*+NAD*CytochromeCytochrome0,+2H*H,OReducing potential (E)Electronacceptor
Electron transport systems Series of electron acceptors Each successive acceptor in system has higher reduction potential Each has greater tendency to receive electrons Moves electrons in one direction