Experiment 9: Extraction andIdentificationofYeast RNAl.Objectives andreguirements(1) Understand the method for extracting RNA from yeast(2) Learn the identification methods for RNA components(3) Learn and master the use of a centrifuge
Experiment 9: Extraction and Identification of Yeast RNA 1. Objectives and requirements (1) Understand the method for extracting RNA from yeast. (2) Learn the identification methods for RNA components. (3) Learn and master the use of a centrifuge
2.PrinciplesMicroorganisms are raw materials for the industrialproduction of nucleic acids, with yeast beingparticularly ideal.This is because yeast nucleic acid mainly consists ofRNA (2.67-10.0%), with very little DNA (0.03-0.514%)The yeast cells are easy to collect, and RNA is easilyseparated
2. Principles Microorganisms are raw materials for the industrial production of nucleic acids, with yeast being particularly ideal. This is because yeast nucleic acid mainly consists of RNA (2.67-10.0%), with very little DNA (0.03-0.514%). The yeast cells are easy to collect, and RNA is easily separated
2.PrinciplesThe commonly used methods in industrial production arethe dilute alkali method and the concentrated salt method- The former utilizes dilute alkali to dissolve the cell wallThis method has a short extraction time, but RNA isunstable under these conditions and prone to decomposition- The latter involves using high-concentration salt to altercell membrane permeability under heating conditionsThis method is easy to master and produces a betterproduct color
2. Principles The commonly used methods in industrial production are the dilute alkali method and the concentrated salt method. - The former utilizes dilute alkali to dissolve the cell wall. This method has a short extraction time, but RNA is unstable under these conditions and prone to decomposition. - The latter involves using high-concentration salt to alter cell membrane permeability under heating conditions. This method is easy to master and produces a better product color
2.PrinciplesTo avoid molecular degradation, the phenol methodcan be used for RNA preparation.The principle is to treat biological materials withphenol to denature proteins, followed by centrifugationThe upper aqueous layer contains all the RNA, whichcan be precipitated with ethanol
2. Principles To avoid molecular degradation, the phenol method can be used for RNA preparation. The principle is to treat biological materials with phenol to denature proteins, followed by centrifugation. The upper aqueous layer contains all the RNA, which can be precipitated with ethanol
2.PrinciplesIn this experiment, the dilute alkali method is used toextract RNA from yeast.The dilute alkali solution can dissolve the cell wall andrelease RNA, which dissolves in the dilute alkali solutionAfter neutralizing the alkali, ethanol can be added toprecipitate the RNA, thereby obtaining crude RNA
2. Principles In this experiment, the dilute alkali method is used to extract RNA from yeast. The dilute alkali solution can dissolve the cell wall and release RNA, which dissolves in the dilute alkali solution. After neutralizing the alkali, ethanol can be added to precipitate the RNA, thereby obtaining crude RNA