9.2 Transcription is catalyzed by RNA polymerase Figure 9.3 Transcription takes place in a bubble,in which RNA is synthesized by base pairing with one strand of DNA in the transiently unwound region. As the bubble progresses, the DNA duplex reforms behind it,displacing the RNA in the form of a single polynucleotide chain. 清菜大当
Figure 9.3 Transcription takes place in a bubble, in which RNA is synthesized by base pairing with one strand of DNA in the transiently unwound region. As the bubble progresses, the DNA duplex reforms behind it, displacing the RNA in the form of a single polynucleotide chain. 9.2 Transcription is catalyzed by RNA polymerase
9.2 Transcription Enzym e movement is catalyzed by RNA polymerase DNA coding strand Rewind ing point Unwinding point Figure 9.4 During transcription,the bubble is maintained within bacterial RNA polymerase, which unwinds and rewinds DNA,maintains ONA template strand the conditions of the partner and template DNA strands,and synthesizes Catalytic site RNA. RNA bind ing site 清菜大当
Figure 9.4 During transcription, the bubble is maintained within bacterial RNA polymerase, which unwinds and rewinds DNA, maintains the conditions of the partner and template DNA strands, and synthesizes RNA. 9.2 Transcription is catalyzed by RNA polymerase
9.2 Transcription is catalyzed by RNA polymerase Figure 9.5 Yeast RNA polymerase has grooves that could be binding sites for nucleic acids. The pink beads show a possible path for DNA that is ~25 A wide and 5-10 A deep.The green beads show a narrower channel. 清菜大当
Figure 9.5 Yeast RNA polymerase has grooves that could be binding sites for nucleic acids. The pink beads show a possible path for DNA that is ~25 Å wide and 5-10 Å deep. The green beads show a narrower channel. 9.2 Transcription is catalyzed by RNA polymerase
5 end of 5'nucleotide 9.2 Transcription triphosphate being added to chain is catalyzed by 0P,0 RNA polymerase Figure 9.6 Phosphodiester bond formation involves a hydrophilic attack by the 0=P0 3'-OH group of the last nucleotide of the chain on OH the 5'triphosphate of the Pyrophosphate is released incoming nucleotide,with release of pyrophosphate 清苇大兰
Figure 9.6 Phosphodiester bond formation involves a hydrophilic attack by the 3’-OH group of the last nucleotide of the chain on the 5’ triphosphate of the incoming nucleotide, with release of pyrophosphate. 9.2 Transcription is catalyzed by RNA polymerase
9.2 Transcription 工 is catalyzed by RNA polymerase Badk and ef enzyme 进进进进进进进 Figure 9.6 RNA polymerase moves like an inchworm during elongation, when it compresses from the back end and release from the front end 清菜大当
Figure 9.6 RNA polymerase moves like an inchworm during elongation, when it compresses from the back end and release from the front end. 9.2 Transcription is catalyzed by RNA polymerase