Chongqing Medical University Curricula Syllabus Course Title: Molecular Biology Major: Overseas students course Types: Theory Course Edited Date: March 16,2012
Chongqing Medical University Curricula Syllabus Course Title: Molecular Biology Major: Overseas students course Types: Theory Course Edited Date: March 16, 2012
《Molecular Biology》curricula syllabus (edited in 2005,revised in 2012) 1.Course Code:015000280 2.English name:Molecular Biology 3.Types of cours s:required course 4.Elective course:Biochemistry,zoology and cell biology 5.Credits: 3 6.Credit hours:90(including 8 hours for experimental hours) 7.Course outline: Molecular biology is compury for students in the major of biotechnology.Molecula biology wants to generate a complete description of the structure,function,and interrelationships of the cell's macromolecules.and thereby to understand why living cells behave the way they do.Its aims and tasks of teaching,is to understand and to master molecular biology's basic theory and technology,to understand the application of molecular biology.Beca and technology of molecular rapi and new technologies and from er lab oratori es nearly everyday,so base on the development of molecular biology discipline,new knowledge and technology will be introduced in the course of our teaching 8.Teaching purposes: 1.Knowing the basic concepts of biolo 2.Understandingth 3.Understanding the history of development of theory,technology and its applications in molecular biology 9.Teaching Methods 1.Lecture:to introduce the concepts and theories in bioloy by and easy compr students to intuitively understand the biological macromolecular structure and the interactions between or among them 3.Discuss teaching:By the way of teachers or students to ask questions,the students will fully and actively 10.Experiments:Ple e see the experi ental curricula 11.basic content of teaching and distribution of times Chapter Content hours Chapter 1 Introduction Chapter 2 Genome structure and function Chapter 3 Gene expression (transcription and translation) Chapter 4 Regulation of gene expression 3 Chapter 5 Molecular Biology of technology Chapter 6 DNA transposition,genetics
1 《Molecular Biology》curricula syllabus （edited in 2005, revised in 2012） 1.Course Code：015000280 2.English name: Molecular Biology 3.Types of courses: required course 4.Elective course: Biochemistry, zoology and cell biology 5.Credits: 3 6.Credit hours: 90(including 8 hours for experimental hours) 7.Course outline: Molecular biology is compulsory for students in the major of biotechnology. Molecular biology wants to generate a complete description of the structure,function,and interrelationships of the cell’s macromolecules, and thereby to understand why living cells behave the way they do. Its aims and tasks of teaching, is to understand and to master molecular biology's basic theory and technology, to understand the application of molecular biology. Because theory and technology of molecular biology developed rapidly, and new technologies and methods are emerging from research laboratories nearly everyday, so based on the development of molecular biology discipline, new knowledge and technology will be introduced in the course of our teaching. 8.Teaching purposes： 1,Knowing the basic concepts of molecular biology. 2,Understanding the content of the molecular biology research. 3,Understanding the history of development of theory, technology and its applications in molecular biology. 9.Teaching Methods 1, Lecture: to introduce the numerous complex concepts and theories in molecular biology by using simple and easy comprehensive sentences or words. 2. Intuitive teaching: to make full use of model teaching and multimedia teaching, to allow students to intuitively understand the biological macromolecular structure and the interactions between or among them. 3. Discuss teaching : By the way of teachers or students to ask questions, the students will fully express their ideas and actively participate in the couse of teaching. 10. Experiments: Please see the experimental curricula. 11. basic content of teaching and distribution of times Chapter Content hours Chapter 1 Introduction 3 Chapter 2 Genome structure and function 3 Chapter 3 Gene expression (transcription and translation) 3 Chapter 4 Regulation of gene expression 3 Chapter 5 Molecular Biology of technology 3 Chapter 6 DNA transposition, genetics 3
Chapter 7 Tumor molecular biology Chapter 8 Cell signal transduction molecular biology Chapter9 Relationship between gene techniques and 3 diseases of mankind total hours of theory teaching 之 Experiment 0 Total hour Chapter 1 Introduction (3 hours) Requirements for teaching:to understand the molecular biology's target,content and methods of molecular biology,to understand theory and history of the development of technology and its Content of teaching Section 1 A brief history of molecular biology 1.what is molecular biology 2.Time clues of the development in molecular biology Section2 1,the molecular biology of DNA 2.the molecular biology of protein 3,the cell signal transduction in molecular biology Section3 Prospects of molecular biology 1,genomics 2.the post-genomics 3,pharmacological genomics Chapter2The genome structure and function(3 hours) Requirements for teaching:to master the basic concepts as follows:gene,the genome broken ene,gene family,silencer. super-gene family,DNA polymorphism,repetitive,DNA methylation. zymes antisens eRNA.protein structure,nuclear RNA and inequality within small nuclear RNA;to be familiar with the prokaryote and eukaryotic genome structure and function,to be familiar with the DNA double helix structure of the original nuclear,eukaryotic mRNA in structural characteristics. Teaching content柱 Section I the ge ome structure and function genes and genome concept 2.the virus genome structure and function 3,the bacterial genome structure and function 4.restriction fragment length polymorphism 5,the mitochondrial DNA structure and function 6.structure 7.genome structure and function Section 2 the DNA molecular structure and function 1 nucleic acid's research history 2.the chemical composition of DNA 3.the pri stru are of DNA 4.acids variability and its
2 Chapter 7 Tumor molecular biology 3 Chapter 8 Cell signal transduction molecular biology 3 Chapter 9 Relationship between gene techniques and diseases of mankind 3 total hours of theory teaching 27 Experiment 9 Total hours 36 Chapter 1 Introduction (3 hours) Requirements for teaching: to understand the molecular biology’s target, content and methods of molecular biology; to understand theory and history of the development of technology and its applications. Content of teaching: Section 1 A brief history of molecular biology 1. what is molecular biology 2. Time clues of the development in molecular biology Section2 research content of molecular biology 1, the molecular biology of DNA 2, the molecular biology of protein 3, the cell signal transduction in molecular biology Section3 Prospects of molecular biology 1, genomics 2, the post-genomics 3, pharmacological genomics Chapter 2 The genome structure and function (3 hours) Requirements for teaching: to master the basic concepts as follows: gene, the genome, broken gene, gene family, silencer, super-gene family, DNA polymorphism, repetitive sequences, DNA methylation, ribozymes, antisense RNA, protein structure, nuclear RNA and inequality within a small nuclear RNA ; to be familiar with the prokaryote and eukaryotic genome structure and function; to be familiar with the DNA double helix structure of the original nuclear, eukaryotic mRNA in structural characteristics. Teaching content: Section 1 the genome structure and function 1, genes and genome concept 2, the virus genome structure and function 3, the bacterial genome structure and function 4, restriction fragment length polymorphism 5, the mitochondrial DNA structure and function 6. Eukaryotic cell chromosome structure 7. Eukaryotic chromosome genome structure and function Section 2 the DNA molecular structure and function 1, nucleic acid’s research history 2, the chemical composition of DNA 3, the primary structure of DNA 4, nucleic acid’s variability and it’s rehabilitation
Chapter 3 Gene expression(3 hours) Teaching requirements:Master of genetic information.transcription translation.meaningful chain,linked transcription template,open reading frames,the genetic code,and other basic concepts;familiar with the prokaryote RNA and protein synthesis of the basic biological process eukaryotic RNA and protein synthesis features understanding transcription after the processing and translation. Teaching content: Section I the biosynthesis of RNA-transcription 1.Introduction 2,RNA polymerase 3.the promoter 4.transcription process 5.RNA processing and maturity 6.RNA editing mechanisms 7.the roe of eukaryotic transcription srto o e Biolesical svahesis oforolintration 1.mRNA and the genetic code 2.deciphered the genetic code 3 tRNA the structure and function 4.rRNA and ribosomal 5.the onof amino acidsand t'stransition 6.the biosynthesis of Prokaryotic protein 7.the biosynthesis of Eukaryotic protein Chapter 4 Regulation of gene expression (3 hours) Teaching requirements:Master gene ne,compose d of gene expre expres .express on a ,the role of trans-factor,tissue-specific expression of the basic concepts,familiar with the eukaryotic gene expression and regulation of various levels.DNA The level of regulation of different wavs the role of trans-factor and the main characteristics of regulation.after the transcription of the different segm nts and ansation level after level of on the basic link in understar ing the role of trans-domai mode and nti-role-acting acto ue-specific gene expression and the phase Teaching content: Section I gene expression and regulation of Prokaryote I the introduce of the Operon 2 the basic of Operon of the peron'sexpressionand 4,Operon of tryptophar Section 2 eukaryotic gene expression and regulation 1.the complexity of the Eukaryotic genome 2.protein phosphorylation. signal tr nsduction and gene expression Chapter5 Molecular biology techniques(3 hours) Teaching requirements:Understanding the basic molecular biology techniques and experimental methods.such as recombinant DNA technology.DNA extraction.cell culture.molecular hybridization,ELISA,nucleic acid sequencing(Sanger-mediated chain termination method),PCR
3 Chapter 3 Gene expression (3 hours) Teaching requirements: Master of genetic information, transcription, translation, meaningful chain, linked transcription template, open reading frames, the genetic code, and other basic concepts; familiar with the prokaryote RNA and protein synthesis of the basic biological process, eukaryotic RNA and protein synthesis features; understanding transcription after the processing and translation. Teaching content: Section I the biosynthesis of RNA - transcription 1. Introduction 2, RNA polymerase 3, the promoter 4, transcription process 5, RNA processing and maturity 6, RNA editing mechanisms 7, the role of eukaryotic transcription 8, antisense RNA Section II of the biological synthesis of protein - translation 1, mRNA and the genetic code 2, deciphered the genetic code 3, tRNA the structure and function 4, rRNA and ribosomal 5, the activation of amino acids and it’s transition 6. the biosynthesis of Prokaryotic protein 7. the biosynthesis of Eukaryotic protein Chapter 4 Regulation of gene expression (3 hours) Teaching requirements:Master gene expression, housekeeper gene, composed of gene expression, induced expression, to curb the expression, coordinated expression, expression and regulation, the role of trans-factor, tissue-specific expression of the basic concepts; familiar with the eukaryotic gene expression and regulation of various levels, DNA The level of regulation of different ways, the role of trans-factor and the main characteristics of regulation, after the transcription of the different segments of control, translation and translation level after level of control the basic link in understanding the role of trans-domain model and anti-role-acting factor , The tissue-specific gene expression and the phase. Teaching content: Section 1 gene expression and regulation of Prokaryote 1,the introduce of the Operon 2, the basic composition of Operon 3, lactose manipulation of the Operon’s expression and regulation 4, Operon of tryptophan Section 2 eukaryotic gene expression and regulation 1. the complexity of the Eukaryotic genome 2, protein phosphorylation, signal transduction and gene expression 3, the hormone and its effects Chapter 5 Molecular biology techniques (3 hours) Teaching requirements: Understanding the basic molecular biology techniques and experimental methods, such as recombinant DNA technology, DNA extraction, cell culture, molecular hybridization, ELISA, nucleic acid sequencing (Sanger-mediated chain termination method), PCR
RT-PCR.etc.the basic principle and its application. Teaching content: 1.the concept of recombinant DNA technology 2.the application of recombinant DNA technology 3.the briefhistory of recombinant DNA technology 4,the development of recombinant DNA technology Events 5.the recombinant DNA technique 6.the determination of DNA sequencing (sanger) 7.protein immune blotting technology Chapter6 DNA Transposable,the introduction of Epigenetics (3hours) Teaching requirements:Understanding the principle of DNA Transposable and its various blocks. apparent understanding of the concept of genetics and genetics research in recent vears.the apparent situation. Teaching content: 1.DNA Transposable 2.Epigenetics Chapter7 Oncomolecularbiology (3 hours) Teaching requirements:Understanding the incidence of cancer gene theory and the level of interpretation. Teaching content: 1,cancer and tumo 2,the cancer gene in the general mechanism 3.the tumors progress on the molecular level Chapter8 Cell signal transduction molecular biology (3 hours) :Understanding the basic moleculr mechanisms and arch progress Teaching content 1.the cell signal transduction process in general 2.cell signal transduction of the main channels 3.cell signaling molecule,the transcription factor abnormalities and disease 4.a number o of signal t stacles apter Gene technology and humar relations(3 hours) Teaching requirements:Understanding the applications of gene technology and applications in many industries in the progress of the case Teaching content: nicplants and agricultural production 2.gene techn y nd h man health 3,the application of the biotechnology industry 12.the main teaching materials and teaching reference books: 1,edited by Feng Zuohua,Qu Shen,Sun Jun Beijing.People's Medical Publishing House. March 2007 the 1st edition 2.edited by ert F.Weaver,molecular biology,Beijing.Science Press August 2000 the first 3.other modern molecular biology approved by:Yi Faping January 8.2005 Author:YiFaping March 16 2012
4 RT-PCR, etc. the basic principle and its application. Teaching content: 1, the concept of recombinant DNA technology 2, the application of recombinant DNA technology 3, the brief history of recombinant DNA technology 4, the development of recombinant DNA technology Events 5, the recombinant DNA technique 6, the determination of DNA sequencing (sanger) 7, protein immune blotting technology Chapter 6 DNA Transposable, the introduction of Epigenetics (3 hours) Teaching requirements:Understanding the principle of DNA Transposable and its various blocks; apparent understanding of the concept of genetics and genetics research in recent years, the apparent situation. Teaching content: 1. DNA Transposable 2. Epigenetics Chapter7 Oncomolecularbiology (3 hours) Teaching requirements: Understanding the incidence of cancer gene theory and the level of interpretation. Teaching content: 1, cancer and tumor 2 ,the cancer gene in the general mechanism 3, the tumors progress on the molecular level Chapter8 Cell signal transduction molecular biology (3 hours) Teaching requirements: Understanding signal transduction the basic molecular mechanisms and channels, and research progress. Teaching content: 1, the cell signal transduction process in general 2, cell signal transduction of the main channels 3, cell signaling molecule, the transcription factor abnormalities and disease 4, a number of areas of signal transduction obstacles Chapter9 Gene technology and human relations (3 hours) Teaching requirements: Understanding the applications of gene technology and applications in many industries in the progress of the case. Teaching content: 1, transgenic plants and agricultural production 2, gene technology and human health 3, the application of the biotechnology industry 12. the main teaching materials and teaching reference books: 1, edited by Feng Zuohua, Qu Shen, Sun Jun Beijing, People’s Medical Publishing House, March 2007 the 1st edition. 2. edited by Robert F. Weaver, molecular biology, Beijing, Science Press, August 2000 the first edition. 3. other modern molecular biology. approved by: Yi Faping January 8,2005 Author: Yi Faping March 16, 2012