B Synchronization of cell(for cell population) (1) Natural synchronization Early embryo in most invertebrates and a few vertebrates Fruit fly embryo 级
B. Synchronization of cell (for cell population) (1) Natural synchronization Early embryo in most invertebrates and a few vertebrates Fruit fly embryo
(2) Selected synchronization artificially Isolation of cells in M phase Isolation of cells by centrifugation
(2) Selected synchronization artificially Isolation of cells in M phase Isolation of cells by centrifugation
3) Synchronization of cells induced by drugs Blocking DNA synthesis by Tdr G1/s--TdR GU/S G2 Blocking cells in metaphase by colchicine Affect the assembly of mitotic spindle
(3) Synchronization of cells induced by drugs Blocking DNA synthesis by TdR G1/S—TdR G1 G2 S M G1/S Blocking cells in metaphase by colchicine Affect the assembly of mitotic spindle
Special cell cycles Early embryo 30min/cell cycle) (1)No GI and g2 phase, all replicons are activated, so S phaseis very short (2)Have little or no need to synthesize components other than dna. cell division 3)No cell growth during cell cycle
Special cell cycles— Early embryo (30min/cell cycle) (1)No G1 and G2 phase, all replicons are activated, so S phaseis very short (2) Have little or no need to synthesize components other than DNA, cell division (3) No cell growth during cell cycle
Frog Xenopus laevis
Frog Xenopus laevis