Chapter 10 Cytoskeleton System 1. Introduction A. Conception of Cytoskeleton_(Narrow sense) A complex network of interconnected microfilaments microtubules and intermediate filaments that extends throughout the cytosol. Microtubules Microfilaments Intermediate filaments
Cytoskeleton System A. Conception of Cytoskeleton (Narrow sense) A complex network of interconnected microfilaments, microtubules and intermediate filaments that extends throughout the cytosol. Chapter 10 Microbubules Microfilamemts Intermediate filaments 1. Introduction
Actin waner false inown a mco mern are two stranded helical polymers of the protein actin. They appear as flexble structures. t ameter of 5-3 nm, that are organised into a van flaments are dispersed throughout the cell, per are.a. Although 二 st highly oncentrated in the corea jus beneath the plasma membrane. 25 mm m Microtubules are long and straight and typically h single mcrotutule-organdring canter (MTOC TLHMIC病 EPILAMENTS afe elaments ate topehke hens with a diameter of around siamen forms a meshwork estee ous family. One type of intermediae bert ore cef-cell 5 Figure 10-2. The three types of protein filaments that form the cytoskeleton
Figure 10-2. The three types of protein filaments that form the cytoskeleton
B Techniques for studying the cytoskeleton o Fluorescent microscopy and Electron microscopy: Immunofluorescence: fluorescently-labeled antibody Fluorescence: microinject into living cells Video microscopy: in vitro motility assay Electron: Triton X-100, Metal replica o Drugs and mutations(about functions) Biochemical analysis (in vitro
B. Techniques for studying the cytoskeleton ❖ Fluorescent microscopy and Electron microscopy : Immunofluorescence: fluorescently-labeled antibody Fluorescence: microinject into living cells Video microscopy: in vitro motility assays Electron: Triton X-100, Metal replica ❖ Drugs and mutations (about functions) ❖ Biochemical analysis(in vitro)
C. The self-assembly and dynamic structure of cytoskeletal filaments Each type of cytoskeletal filament is constructed from smaller protein subunits. The cytoskeleton is a network of three filamentous structures o The cytoskeleton is a dynamic strucrure with many roles
C. The self-assembly and dynamic structure of cytoskeletal filaments ❖Each type of cytoskeletal filament is constructed from smaller protein subunits. ❖The cytoskeleton is a network of three filamentous structures. ❖The cytoskeleton is a dynamic strucrure with many roles
2. Microfilament MF A MFS are made of actin and involved in cell motility. o Using atP, g-actin polymerizes to form MF(F-actin) s on actin molecule NH COOH ATP (ADP when minus end
2. Microfilament, MF A. MFs are made of actin and involved in cell motility. ❖Using ATP, G-actin polymerizes to form MF(F-actin)