Chapter 3. Techniques in Cell Biology Preparatory observe put forward theoretics Design control tests Refer to knowledge Collect data Explain results Devise conclusion
Chapter 3. Techniques in Cell Biology Preparatory observe put forward theoretics Design control tests Collect data Explain results Devise conclusion Refer to knowledge
从整个生命科学的发展趋势看细胞 生物学方法 分子水平 细胞水平 结构功能 细胞生命活动 分析 综合 功能基因组学研究是细胞生物学研究的 基础与归宿 (生命科学研究的核心问题)
从整个生命科学的发展趋势看细胞 生物学方法 • 分子水平 细胞水平 • 结构功能 细胞生命活动 • 分析 综合 • 功能基因组学研究是细胞生物学研究的 基础与归宿 （生命科学研究的核心问题）
细胞生物学研究方法 显微观篝 细胞培养 分子生物学技术 组纵学找术 胚胎学技术 生物化学找术 物理学技术
IThe light microscopy Ocular eyepiece Reman fes the image foxmed by the ob ective lens aL lens Body tube Transmis Line ot vision ho image from the ob- ectve iens to the ocular Path of light Objective lenses Prmary Body tube lenses tha! magn ly the spec men Objective Stage Holds the eases in position Condenser Focuses ght througn specimen Condenser anses aphragm Controls the amount of light entering the condenser nato Coarse focusing knob Luminator Lignt source Base wih source of Base (a) Principal parts and functions (b) The path of light(bottom to top) Figure A-6 The Compound Light Microscope. (a)A compound light microscope. (b) The path of light through the compound microscope
1.The Light Microscopy
A. Res solution and magnification 100um animal Figure 3-1. Resolving power. Sizes 1o um of cells and their components drawn on a logarithmIc scale, indicating the range of objects that can be readily resolved by the naked eye and in the light and electron 2100nm virus microscopes. The following units of length are commonly employed in globula microscopy: um(micrometer)=10 6 mnm(nanometer)=10-9m A 1 nm sna (Angstrom unit)=10-10 m atom 0.1nm
Figure 3-1. Resolving power. Sizes of cells and their components drawn on a logarithmic scale, indicating the range of objects that can be readily resolved by the naked eye and in the light and electron microscopes. The following units of length are commonly employed in microscopy: µm (micrometer) = 10- 6 m nm (nanometer) = 10-9 m Å (Ångström unit) = 10-10 m