What is Bacillus subtilis?
What is What is Bacillus acillus subtilis ubtilis?
B. subtilis GenotypicDiversityIl. B. subtilis PhenotypicDiversity
I. B. subtilis Genotypic Diversity ? II. B. subtilis Phenotypic Diversity
Why Bacillus subtilis?
Why Bacillus subtilis? • Well studied • Non-pathogen • Ubiquitous & relatively easy to isolate
Where In the World Is Bacillus subtilis?Hyalite Reservoir,Marburg,GermanySalzburg,AustriaMontanaMoscow,RussiaSt.JohnAlaska(MendenhallGlacier)U.S.DesertsDeathValleySonoranMojaveMoabJapan (natto)HawaiiGuatemalaXinzheng.ChinaColombiaNefta,TunisiaMediterranean SeaMumbai,IndiaVenezuelaNamibiaSahara DesertCoastofEgyptNamibDesert
St. John Moscow, Russia U. S. Deserts Death Valley Sonoran Mojave Moab Nefta, Tunisia Sahara Desert Mediterranean Sea Coast of Egypt Japan (natto) Hyalite Reservoir, Marburg, Germany Montana Guatemala Colombia Venezuela Alaska (Mendenhall Glacier) Xinzheng, China Mumbai, India Hawaii Salzburg, Austria Namibia Namib Desert Where In the World Is Bacillus subtilis?
B. subtilis lsolation:Heatsoilsample (10μg-500mg) Plate for colonies, colony purify 3x (all morphotypes)·MultiplexPCRwith"species-specific"16SrRNAprimers
• Heat soil sample (10 µg - 500 mg) • Plate for colonies, colony purify 3X (all morphotypes) • Multiplex PCR with “species-specific” 16S rRNA primers B. subtilis Isolation