Nick oH② RNA or dna The 5 to 3 exonuclease 3 and polymerase activities Template DNA strand DNA of DNA polymerase I polymerase I OH P allows the enzyme to catalyze the nick dNMPs ANTP. 32P-dATP translation process TAMPs (PP;) of a dna or rna fragment base paired 5 3 with a template. ● Nick
The 5` to 3` exonuclease and polymerase activities of DNA polymerase I allows the enzyme to catalyze the nick translation process of a DNA or RNA fragment base paired with a template. 32P-dATP
Arthur Kornberg won the 1959 Nobel prize in Medicine for his discovery of the mechanism in the biological synthesis of deoxyribonucleic acid (before Watson and Crick won theirs!)
Arthur Kornberg won the 1959 Nobel Prize in Medicine for his discovery of the mechanism in the biological synthesis of deoxyribonucleic acid (before Watson and Crick won theirs!)
8. The synthesis of the two daughter DNA Strands was found to be semidiscontinuous At a replication fork, the overall elongation direction for one daughter strand is 5 to 3 and 3 to 5 for the other due to the antiparallel features of dNa duplexes DNA polymerase catalyzing 3 to 5 extension was hypothesized but never identified Reiji okazaki discovered( in the 1960s)that a significant proportion of newly synthesized DNA exists as small fragments
8. The synthesis of the two daughter DNA strands was found to be semidiscontinuous • At a replication fork, the overall elongation direction for one daughter strand is 5` to 3` and 3` to 5` for the other due to the antiparallel features of DNA duplexes. • DNA polymerase catalyzing 3` to 5` extension was hypothesized but never identified. • Reiji Okazaki discovered ( in the 1960s) that a significant proportion of newly synthesized DNA exists as small fragments!
These So-called Okazaki fragments was found to join together by DNA ligases to form one of the daughter strands Thus both daughter strands are synthesized in 5 to 3 direction One daughter strand at the replication fork is synthesized continuously and the other discontinuously, called the leading and lagging strands respectively
• These so-called Okazaki fragments was found to join together by DNA ligases to form one of the daughter strands; • Thus both daughter strands are synthesized in 5` to 3` direction. • One daughter strand at the replication fork is synthesized continuously and the other discontinuously, called the leading and lagging strands respectively
Overall direction of progeny chain growth 5 at a replicating fork: one in 53 and the other in 3-5' direction 3 5× 3 5 53
Overall direction of progeny chain growth at a replicating fork: one in 5’ 3’ and the other in 3’ 5’ direction