REPLICATED DNA 加论 plicated,4 L3 久 positiressuperco L2 p的 Replicating SV40 DNA No complete unwinding of chains!
Replicating SV40 DNA Unreplicated, positive supercoils of parent strands Replicated DNA No complete unwinding of chains!
Autoradiogram of a replicating E coli chromosomal dna labeled by H thymidine. No complete unwinding of f DNA chains!
Autoradiogram of a replicating E.coli chromosomal DNA labeled by [3H]thymidine. No complete unwinding of DNA chains!
5. DNA replication was found to begin at specific sites and proceed bidirectionally Pulse-chase labeling studies of replicating DNA, as well as direct eM examination of intermediates of replicating linear T7 bacteriophage DNA all revealed that DNa replication is bidirectional Denaturation mapping studies with a series of replication intermediates of circular DNa and direct observation of a linear dna revealed that dna replication begins at specific replication origins
5. DNA replication was found to begin at specific sites and proceed bidirectionally • Pulse-chase labeling studies of replicating DNA, as well as direct EM examination of intermediates of replicating linear T7 bacteriophage DNA all revealed that DNA replication is bidirectional. • Denaturation mapping studies with a series of replication intermediates of circular DNA and direct observation of a linear DNA revealed that DNA replication begins at specific replication origins
Replication bubble GF Autoradiogram of replicating mammalian cellular DNA pulse-chase labeled with SHIthymine: replication is bidirectional
Autoradiogram of replicating mammalian cellular DNA pulse-chase labeled with [ 3H]thymine: replication is bidirectional
Examination of T7 DNA (linear) replication using electron microscopy 1. The daughter polynucleotide strands are synthesized almost as soon as the parental strands separate(no complete unwinding of chains) 2. Replication always began at a specific internal site(not from the ends. not random) 3. The replication proceeds in both directions(determined by measuring the distance between the replication fork and the ends) Replication g
Examination of T7 DNA (linear) replication using electron microscopy: 1. The daughter polynucleotide strands are synthesized almost as soon as the parental strands separate (no complete unwinding of chains); 2. Replication always began at a specific internal site (not from the ends, not random); 3. The replication proceeds in both directions (determined by measuring the distance between the replication fork and the ends) Replication origin Replication forks