What have you heard about enzymes? 2
What have you heard about enzymes? 2
Think about your breakfast If eaten,digested in hours; If not eaten,stay for years..... 3
Think about your breakfast If eaten, digested in hours; If not eaten, stay for years….. 3
Enzymes are biological catalysts Enzymes affect reaction rates,not equilibria. HO Orotate decarboxylase Kat=39S-1→25×103 second Kon=2.8×1016S-1→78×105year Kcat/knon=1.4×1017 4
4 Orotate decarboxylase Knon = 2.8 10-16 S -1 ➔ 78 106 year Kcat= 39 S-1 ➔ 25 10-3 second Kcat / knon = 1.4 1017 Enzymes are biological catalysts. Enzymes affect reaction rates, not equilibria
Most enzymes are proteins. Could be monomeric or oligomeric enzymes; Could be simple or conjugated enzymes; holoenzyme=apoenzyme cofactor 可 Table 3.1 for the most important coenzymes. 5
Most enzymes are proteins. Could be monomeric or oligomeric enzymes; Could be simple or conjugated enzymes; holoenzyme = apoenzyme + cofactor Table 3.1 for the most important coenzymes. 5
Chymotrypsin (PDB 7GCH)
6 Chymotrypsin (PDB 7GCH)