Chymotrypsin Active site 7
7 Chymotrypsin Active site
Chymotrypsin hydrophobic substrate pocket Catalytic triad:Ser195,His57,Asp102. Inhibitor
8 Catalytic triad: Ser195, His57, Asp102. Inhibitor Chymotrypsin hydrophobic substrate pocket
Chymotrypsin VNQHLCGSHLVEALYLVCGERG Substrate chymotrypsin VNQHLCGSHLVEALY LVCGERG Product ....NH-CH-COOH NH2-CH-CO-.... CH2-CsHa-OH CH2-CH-(CH3)2 9
9 VNQHLCGSHLVEALYLVCGERG VNQHLCGSHLVEALY LVCGERG chymotrypsin ….NH-CH-COOH CH2 -C6H4 -OH NH2 -CH-CO-…. CH2 -CH-(CH3 )2 Substrate Product Chymotrypsin
Mechanism of chymotrypsin (juang.bst.ntu.edu.tw/BCbasics/Animation1.htm) 10
10 Mechanism of chymotrypsin (juang.bst.ntu.edu.tw/BCbasics/Animation1.htm)
Enzymes are potent. Turnover number(kat):the number of substrate molecules converted to product by one enzyme molecule per second when the enzyme is fully saturated. Enzyme Turnover Number [sec]* Carbonic anhydrase 600,000 Catalase 80,000 Acetylcholinesterase 25,000 Triose phosphate isomerase 4,400 a-Amylase 300 Lactate dehydrogenase(muscle) 200 Chymotrypsin 100 Aldolase 11 Lysozyme 0.5 Fructose 2,6-bisphosphatase 0.1 11
Enzymes are potent. 11 Turnover number (kcat): the number of substrate molecules converted to product by one enzyme molecule per second when the enzyme is fully saturated