Protein determinationBradfordAssayLowry Method11
11 Protein determination ✓ Bradford Assay ✓ Lowry Method ✓
Oxygen consumption rate (OCR) andextracellular acidification rate (ECAR耗氧率（OCR）和细胞外酸化率（ECAR)12
12 Oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) 耗氧率（OCR）和细胞外酸化率（ECAR）
Oxidative phosphorylation ofmitochondria2009/0711818:1813Clark oxygen electrode (Hansatech, UK)
Clark oxygen electrode 13 (Hansatech, UK) Oxidative phosphorylation of mitochondria
Electron transport chainCytc胞液侧eCytoplasmicosideoooom00YYXYXYYYYYYXYe线粒体内膜DeMtintimaeiIVIIY丫YYI基质侧延胡索酸H20NADH+H+Matrix sideFumaric acid1/20,+2H+NAD+琥珀酸Succinic acid14
14 胞液侧 Cytoplasmic side 基质侧 Matrix side 线粒体内膜 Mt intima Electron transport chain Q 1/2O2+2H+ H2O Ⅲ Ⅰ Ⅱ Ⅳ 延胡索酸 Fumaric acid 琥珀酸 Succinic acid e - Cytc e - e - e - e - NADH+H+ NAD+
ProtongradientCyanideAzideCarbonAntimycinmonoxideVLComplexIComplex IISuccinateCyteYYOcoenzyneQoxidasereductaseJONADHCoenzymeQcoenzymesytochromereductasereductaseATPComplex IIIsynthase1O,+2HSuccinate,ORotenoneThenoyltri.PtericidinfluoroacetoneNADHDCCDAmytalMercurialsCarboxinOligomycinDemerolUncouplers:2.4-dinitrophenolDicumarolFCCP
15 一. 氧化磷酸化的机理 F1-F0-ATPase 复 合 物