Mt Isolation53[6]MAMMALIANBRAINMITOCHONDRIA10%Brainhomogenate2000gx3minpelletsupernotant12,500gx8minCrudemitochondrialpellet(CM)Cresuspendin3%Ficol3%Ficoll6%Ficollresuspendinisolationmedium11,500gx30min11,500gx10minfreemitochondria(CandNM);rn
6 Mt Isolation
General Points for Mt preparations Performance at 4oC on ice. Cell rupturing by mechanical and /orchemical means and resuspension inisolation bufferDifferential centrifugation（差速离心）Storage. Criteria of purity and intactness?
7 General Points for Mt preparations • Performance at 4oC on ice • Cell rupturing by mechanical and /or chemical means and resuspension in isolation buffer • Differential centrifugation（差速离心） • Storage • Criteria of purity and intactness
Equipments for Mt Isolation· Tissue aliquot8
8 • Tissue aliquot Equipments for Mt Isolation
Homogenator 均质机MPOLYTRON9
9 Homogenator 均质机
Centrifuge离心机10
10 Centrifuge 离心机