Electron Microscopy of replicating DNA reveals replicating bubbles. How does one prove bidirectional fork movement?
Electron Microscopy of replicating DNA reveals replicating bubbles. How does one prove bidirectional fork movement?
Pulse with radiolabeled nucleotide: chase with cold nucleotide. Then do autoradiography a)Predicted fiber autoradiographic pattern Hot Warm Unidirectional growth Bidirectional growth b) Actual fiber autoradiographic pattern Or-
Pulse with radiolabeled nucleotide; chase with cold nucleotide. Then do autoradiography
DNA Replication DNA replication is semi-conservative, one strand serves as the template for the second strand Furthermore, dNa replication only occurs at a specific step in the cell cycle The following table describes the cell cycle for a hypothetical cell with a 24 hr cycle Stage Activ ity Duration G1 Growth and increase in cell size 10 hr DNA synthesis 8 hr G2 Post-DNA synthesis 5 hi Mitosis DNA replication has two requirements that must be met DNA template 2 Free 3-OH group
DNA Replication DNA replication is semi-conservative, one strand serves as the template for the second strand. Furthermore, DNA replication only occurs at a specific step in the cell cycle. The following table describes the cell cycle for a hypothetical cell with a 24 hr cycle. Stage Activity Duration G1 Growth and increase in cell size 10 hr S DNA synthesis 8 hr G2 Post-DNA synthesis 5 hr M Mitosis 1 hr DNA replication has two requirements that must be met: 1. DNA template 2. Free 3' -OH group
(A)Dispersive (B) Conservative Parent double helix ■■■■■■■■■■ (c)Conservative KEY TT Parent DNa TTTT New DNA
DNA Replication DNA replication is semi-conservative, one strand serves as the template for the second strand Furthermore, dNA replication only occurs at a specific step in the cell cycle The following table describes the cell cycle for a hypothetical cell with a 24 hr cycle Stage Activ ity Duration G1 Growth and increase in cell size 10 hr DNA synthesis 8 hr G2 Post-DNA synthesis 5 hr M Mitosis DNA replication has two requirements that must be met DNA template 2 Free3′- OH group
DNA Replication DNA replication is semi-conservative, one strand serves as the template for the second strand. Furthermore, DNA replication only occurs at a specific step in the cell cycle. The following table describes the cell cycle for a hypothetical cell with a 24 hr cycle. Stage Activity Duration G1 Growth and increase in cell size 10 hr S DNA synthesis 8 hr G2 Post-DNA synthesis 5 hr M Mitosis 1 hr DNA replication has two requirements that must be met: 1. DNA template 2. Free 3' -OH group