Facts(“ vocabulary Concepts Techniques Quantitation
Facts (“vocabulary”) Concepts Quantitation Techniques
In vivo dna binding pattern of the polycomb Txn factor What are the genes to which it binds? 2. How does it affect these genes? 3. hat determines where it Binds?? FIG3 Polycomb-binding sites. The picture shows the association of the Polycomb protein with specific sites of the Drosopbila genome, revealed by immunostaining salivary gland pOlytene chromosomes with antibodies against the Polycomb protein(Preparation by
In vivo DNA binding pattern of the Polycomb Txn Factor 1. What are the genes to which it binds? 2. How does it affect these genes? 3. What determines where it Binds??
O-P=0 H2CO Base Nitrogenous base HH Sugar o H hosphate Hc Bas H H HC ase HH o H
1 Nitrogenous base Sugar Phosphate
Evidence for the double helix 1. Fiber Diffraction data helical helical geometry 10 laver 34A° spacing(1A°=1010m) L ines 34A° pitch Between Patterns 2. Structure of dcTP 10 Residues 3. Base tautomerism Per turn) 3. Chargaff rules A=TG=C IGURE 4.9 Evidence for the structure of DNA. this photograph, taken by Rosalind Franklin, shows the x-ray diffraction pattern produced by wet DNA fibers. It played a key role in the lucida tion of DNA structure. The cross pattern indi- cates a helical structure, and the strong spots at top and bottom correspond to a helical rise of 0. 34 nm. The layer line spacing is one-tenth of the distance from the center to either of IA these spots, showing that there are 10 base pairs per repeat
1A 2. Structure of dCTP 3. Base Tautomerism 3. Chargaff rules - A=T, G=C helical 10 layer Lines Between Cross Patterns (10 Residues Per turn) Evidence for the Double Helix 1. Fiber Diffraction data: -Helical geometry -3.4 A º spacing (1Aº = 10-10 m) -34 A º pitch
NIH (not in handout)
NIH (not in handout)