Sequencing technology:lluminaondgenerationsequencing,sequencingbysynthesis1.PrpGAttathONAte surhat3.BidgeArepificatshruet FiaBstecrintl生物信息学2017/10/106/25
Sequencing technology: Illumina 2 nd generation sequencing, sequencing by synthesis. 周宇 ⽣物信息学 2017/10/10 6 / 25
Sequencingtechnologyapplication3.53.0AREMRRACPOZ--1.5DRNCAOErFracARSsui201320062007200820092010201120122014Figure4,OverviewofSelectedHTSApplicationsPbicationdaerpreertataticescribingamodersuseumbrcitaonsthattheaticleclived,Methodsarecolrdbycaoryansizeofthedatapointisproportioraltopubicationrate (citations/monthaj,.Theeetindicatesrecolorkeyasweltheproportionofmeods ineachgroup,Forclarity,seq hasbeen omited from the labels.生物信息学2017/10/107/25同字
Sequencing technology application 周宇 ⽣物信息学 2017/10/10 7 / 25
Sequencing technology application。基因组测序Genome（cancers）功能元件FunctionalElements·表观基因组学Epigenome转录组学Transcriptome·三/四维基因组3DGenome同宇生物信息学2017/10/108/25
Sequencing technology application 基因组测序 Genome (cancers) 功能元件 Functional Elements 表观基因组学 Epigenome 转录组学 Transcriptome 三/四维基因组 3D Genome 周宇 ⽣物信息学 2017/10/10 8 / 25
New reguirements in sequencing era.GenomeassemblyMappingbillionsofshortreadsontogenome。Gene predictionGeneexpressionquantificationAlternativesplicingidentification and quantificationTFs/RBPsbindingsiteidentificationMotifdiscoveryforTFsandRBPsDifferential expression,differential bindingModificationidentificationDNA/Histones/RNAAl/Machine Learning同字生物信息学2017/10/109/25
New requirements in sequencing era Genome assembly Mapping billions of short reads onto genome Gene prediction Gene expression quantification Alternative splicing identification and quantification TFs/RBPs binding site identification Motif discovery for TFs and RBPs Differential expression, differential binding Modification identification DNA/Histones/RNA AI / Machine Learning 周宇 ⽣物信息学 2017/10/10 9 / 25