上浒充通大￥ SHANGHAI JIAO TONG UNIVERSITY 40 TONG 1896 1920 1987 2006 When,where,how much should protein be synthesized? Lecture 8-1 Regulation of gene expression CHAPTER 8 in BROCK BIOLOGYOFMICROORGANISMS Chen Feng TONG UN School of Life Science and Technology Shanghai Jiao Tong University Email:cf2001@sjtu.edu.cn http://micro.sjtu.edu.cn
1896 1920 1987 2006 Lecture 8-1 Regulation of gene expression When, where, how much should protein be synthesized? Chen Feng School of Life Science and Technology Shanghai Jiao Tong University Email: cf2001@sjtu.edu.cn http://micro.sjtu.edu.cn CHAPTER 8 in BROCK BIOLOGY OF MICROORGANISMS
上游充通大￥ SHANGHAI JIAO TONG UNIVERSITY 1896 1920 1987 2006 What is true for E colf is also true for elephant,only more so acAI11A00AV/八I/rnc wwwwwn
1896 1920 1987 2006 What is true for E. coli is also true for elephant, only more so
上游充通大 SHANGHAI JIAO TONG UNIVERSITY Example:Can E.col/cells choose different kinds of foods? If glucose and lactose are Growth on both available,E.coli will lactose Glucose utilize glucose first. exhausted Only after all glucose has been used up,can lactose be used as carbon source. Only after glucose has been llao anneley Growth on used up,B-galactosidase glucose will be produced B-半乳糖 苷酶 0 1 2 3 4 Time(h) 02012R Diauxic growth on a mixturelof glucoseianddactose3iotech
CHEN FENG，School of Life Sciences and Biotech. Example: Can E. coli cells choose different kinds of foods? If glucose and lactose are both available, E. coli will utilize glucose first. Only after all glucose has been used up, can lactose be used as carbon source. Only after glucose has been used up, -galactosidase will be produced -半乳糖 苷酶 Diauxic growth on a mixture of glucose and lactose
上浒充通大学 SHANGHAI JIAO TONG UNIVERSITY TONG I.Overview of Regulation 8.1 Major modes of regulation Regulation is the change in amount and activity of proteins(enzymes) Scarce protein-10 copies Abundant protein-100,000 copies Rate of synthesis of proteins can change over 1000 fold range Activity change of enzymes CHEN FENG,School of Life Sciences and Biotech
CHEN FENG，School of Life Sciences and Biotech. I. Overview of Regulation 8.1 Major modes of regulation Regulation is the change in amount and activity of proteins (enzymes) • Scarce protein-10 copies • Abundant protein-100,000 copies • Rate of synthesis of proteins can change over 1000 fold range • Activity change of enzymes
上浒充通大淫 SHANGHAI JIAO TONG UNIVERSITY Major Modes of Regulation Two major levels of regulation in the cell: One controls the activity of preexisting enzymes Posttranslational regulation Very rapid process (seconds) 。 One controls the amount of an enzyme Regulate level of transcription Regulate translation Slower process (minutes) CHEN FENG,School of Life Sciences and Biotech
CHEN FENG，School of Life Sciences and Biotech. Major Modes of Regulation Two major levels of regulation in the cell: • One controls the activity of preexisting enzymes • Posttranslational regulation • Very rapid process (seconds) • One controls the amount of an enzyme • Regulate level of transcription • Regulate translation • Slower process (minutes)