上游充通大 SHANGHAI JIAO TONG UNIVERSITY 1896 1920 1987 2006 Virus:Growth,quantification,replication and diversity Lecture 9-2 Essentials of Virology CHAPTER 9 in BROCK BIOLOGY OF MICROORGANISMS Chen FengNG School of Life Science and Biotechnology, Shanghai Jiao Tong University Email:cf2001@situ.edu.cn http://micro.sjtu.edu.cn
1896 1920 1987 2006 Lecture 9-2 Essentials of Virology Virus: Growth, quantification, replication and diversity CHAPTER 9 in BROCK BIOLOGY OF MICROORGANISMS Chen Feng School of Life Science and Biotechnology, Shanghai Jiao Tong University Email: cf2001@sjtu.edu.cn http://micro.sjtu.edu.cn
上游充通大￥ SHANGHAI JIAO TONG UNIVERSITY lI.Viral Replication病毒复制 9.4 General Features of Virus Replication 9.5 Virus Multiplication:Attachment and Penetration 9.6 Virus Multiplication:Production of Virual Nucleic Acid and Protein Chen Feng,Shanghai Jiao Tong University
Chen Feng, Shanghai Jiao Tong University II. Viral Replication病毒复制 9.4 General Features of Virus Replication 9.5 Virus Multiplication: Attachment and Penetration 9.6 Virus Multiplication: Production of Virual Nucleic Acid and Protein
上游充通大学 SHANGHAI JIAO TONG UNIVERSITY 9.4 General Features of Virus Replication Phases of Viral Replication Attachment(adsorption)of the virus to a susceptible host cell Entry (penetration)of the virion or its nucleic acid Synthesis of virus nucleic acid and protein by cell metabolism as redirected by virus Assembly of capsids and packaging of viral genomes into new virions(maturation) Release of mature virions from host cell Chen Feng,Shanghai Jiao Tong University
Chen Feng, Shanghai Jiao Tong University 9.4 General Features of Virus Replication Phases of Viral Replication • Attachment (adsorption) of the virus to a susceptible host cell • Entry (penetration) of the virion or its nucleic acid • Synthesis of virus nucleic acid and protein by cell metabolism as redirected by virus • Assembly of capsids and packaging of viral genomes into new virions (maturation) • Release of mature virions from host cell
Figure 9.8 Virion DNA Attachment (adsorption) Cell (host) Protein coat remains outside Penetration (injection) Viral DNA enters Synthesis of nucleic acid and protein Assembly and packaging Release (lysis) Virions 2012 Pearson Education,Inc
Figure 9.8 Protein coat remains outside Viral DNA enters Virion DNA Cell (host) Virions Attachment (adsorption) Penetration (injection) Synthesis of nucleic acid and protein Assembly and packaging Release (lysis) © 2012 Pearson Education, Inc
Following adsorption,the Eclipse Maturation infectivity of the virus particles disappears,a Early Nucleic Protein phenomenon enzymes acid coats called eclipsel隐蔽 期 During the latent Virus period潜伏期， added Assembly replication of viral and nucleic acid and Latent period release protein occurs.The maturation period follows(virus nucleic acid and protein are Time assembled) Figure 9-9 Brock Biology of Microorganisms 11/e 2006 Pearson Prentice Hall,Inc. One-step growth curve一步生长曲线
One-step growth curve 一步生长曲线 Following adsorption, the infectivity of the virus particles disappears, a phenomenon called eclipse隐蔽 期. During the latent period潜伏期, replication of viral nucleic acid and protein occurs. The maturation period follows (virus nucleic acid and protein are assembled)