上泽充通大￥ Shanghai Jiao Tong University Lecture 5-2 Microbial Growth CHAPTER 5 in BROCK BIOLOGY OF MICROORGANISMS Zhao Liping,Chen Feng School of Life Science and Biotechnology Shanghai Jiao Tong University http://micro.sjtu.edu.cn
Lecture 5-2 Microbial Growth Zhao Liping, Chen Feng School of Life Science and Biotechnology Shanghai Jiao Tong University http://micro.sjtu.edu.cn CHAPTER 5 in BROCK BIOLOGY OF MICROORGANISMS
上帝充通大睾 Shanghai Jiao Tong University III.Measuring Microbial Growth 微生物生长的测定（计数）
III. Measuring Microbial Growth 微生物生长的测定（计数）
上降文通大学 Shanghai Jiao Tong University Growth measurements How to count cell numbers in a population Direct count of microbial growth: ·Total cell count,总菌数记数法 ·Counting chambers记数器 ·Viable count活菌记数法 ·Plate counting平板记数 Indirect count of microbial growth:Turbidimetric measurement浊度记数法 ·Photometer分光计 No.3 Shanghai Jiao Tong University
No.3 Shanghai Jiao Tong University Growth measurements How to count cell numbers in a population Direct count of microbial growth: • Total cell count总菌数记数法 • Counting chambers记数器 • Viable count活菌记数法 • Plate counting平板记数 Indirect count of microbial growth: Turbidimetric measurement浊度记数法 • Photometer分光计
上泽充通大学 Shanghai Jiao Tong University 5.7 Direct measurements of microbial growth:total and viable counts Total and viable counting methods each have their strengths and weaknesses.总菌计数与活菌计数法各有利弊。 No.4 Shanghai Jiao Tong University
No.4 Shanghai Jiao Tong University 5.7 Direct measurements of microbial growth: total and viable counts Total and viable counting methods each have their strengths and weaknesses.总菌计数与活菌计数法各有利弊
上泽充通大学 Shanghai Jiao Tong University 5.7.1 Direct Measurements 1:Total cell counts Direct microscopic count:The number of cells in a population can be measured by counting a sample under the microscope.直接显微计数法， With liquid samples,special counting chambers must be used.液体样品需使用计数室/血球计数板 No.5 Shanghai Jiao Tong University
No.5 Shanghai Jiao Tong University 5.7.1 Direct Measurements 1: Total cell counts Direct microscopic count :The number of cells in a population can be measured by counting a sample under the microscope.直接显微计数法, With liquid samples, special counting chambers must be used. 液体样品需使用计数室/血球计数板