The product of gene A is enzyme A,which is synthesized constitutively and carries out its reaction.Enzyme B is also synthesized constitutively but its activity can be inhibited.The synthesis of the product of gene C can be prevented by control at the level of translation.The synthesis of the product of gene D can be prevented by control at the level of transcription Regulate Regulate enzyme synthesis enzyme activity At translation At transcription Substrate Product No product Enzyme A Enzyme B No enzyme Translation 1米 No mRNA Transcription 米 ene
An overview of the mechanisms that can be used in regulation The product of gene A is enzyme A, which is synthesized constitutively and carries out its reaction. Enzyme B is also synthesized constitutively but its activity can be inhibited. The product of gene A is enzyme A, which is synthesized constitutively and carries out its reaction. Enzyme B is also synthesized constitutively but its activity can be inhibited. The synthesis of the product of gene C can be prevented by control at the level of translation. The product of gene A is enzyme A, which is synthesized constitutively and carries out its reaction. Enzyme B is also synthesized constitutively but its activity can be inhibited. The synthesis of the product of gene C can be prevented by control at the level of translation. The synthesis of the product of gene D can be prevented by control at the level of transcription
上游充通大￥ SHANGHAI JIAO TONG UNIVERSITY II.Regulation of Enzyme Activity (Posttranslational level regulation) 8.2 Noncovalent enzyme inhibition酶活 的非共价抑制 CHEN FENG,School of Life Sciences and Biotech
CHEN FENG，School of Life Sciences and Biotech. II. Regulation of Enzyme Activity (Posttranslational level regulation) 8.2 Noncovalent enzyme inhibition 酶活 的非共价抑制
上游充通大学 SHANGHAI JIAO TONG UNIVERSITY 8.2.1 Feedback inhibition反馈抑制 Feedback inhibition Starting material occurs primarily in the regulation of entire Enzyme A biosynthetic pathways First intermediate The activity of the first Enzyme enzyme of the pathway is inhibited by the end Second intermediate product,thus controlling Enzyme production of end Third intermediate product Enzyme End product
CHEN FENG，School of Life Sciences and Biotech. 8.2.1 Feedback inhibition反馈抑制 Feedback inhibition occurs primarily in the regulation of entire biosynthetic pathways The activity of the first enzyme of the pathway is inhibited by the end product, thus controlling production of end product
Mechanism:Allostery变构 Enzyme Allosteric site Substrate-binding site An allosteric enzyme Allosteric Substrate effector has the Active site, Conformational change in substrate-binding site; Enzyme reaction where the substrate enzyme reaction proceeds inhibited binds,and the Allosteric site,where Substrate the inhibitor binds cannot bind reversibly.活性位点 No enzyme reaction Enzyme reaction 与变构位点
Mechanism: Allostery 变构 An allosteric enzyme has the Active site, where the substrate binds, and the Allosteric site, where the inhibitor binds reversibly.活性位点 与变构位点