泽克廷大学 上 Shanghai Jiao Tong University 1896 1920 1987 2006 Lecture 7-3 Principles of Microbial Molecular Biology Chapter 6 in BROCK BIOLOGY OF MICROORGANISMS JIAO TONG Chen Feng School of Life Science and Technology Shanghai Jiao Tong University http://micro.sjtu.edu.cn Email:cf2001@sjtu.edu.cn
1896 1920 1987 2006 Lecture 7-3 Principles of Microbial Molecular Biology Chen Feng School of Life Science and Technology Shanghai Jiao Tong University http://micro.sjtu.edu.cn Email: cf2001@sjtu.edu.cn Chapter 6 in BROCK BIOLOGY OF MICROORGANISMS
上泽充通大学 Shanghai Jiao Tong University Transcription:Discuss the following questions 1.Why do we need a messenger RNA? 2.RNA polymerase:Any direction?Why? 3.RNA polymerase:Need a primer? 4.Initiation of RNA synthesis:How to find the right place? Where is the right place?Which DNA strand is the right strand for transcription? 5.Termination of RNA synthesis:where is the right place for termination? 6.Transcription:write down the relative position of promoter position,terminator,start codon,stop codon for a polycistronic mRNA No. 2 Chen Feng.Shanghai Jiao Tong University
No. 2 Chen Feng, Shanghai Jiao Tong University Transcription: Discuss the following questions 1. Why do we need a messenger RNA? 2. RNA polymerase: Any direction? Why? 3. RNA polymerase: Need a primer? 4. Initiation of RNA synthesis: How to find the right place? Where is the right place? Which DNA strand is the right strand for transcription? 5. Termination of RNA synthesis: where is the right place for termination? 6. Transcription: write down the relative position of promoter position, terminator, start codon, stop codon for a polycistronic mRNA
上泽充通大￥ Shanghai Jiao Tong University IV mRNA Transcription Why do we need a messenger RNA? ·The genetic information needs to be read“one sentence at a time",just like we speak in sentences.Genes are“sentences”in genetic information Protein synthesis takes place away from where chromosome exists No.3 Chen Feng.Shanghai Jiao Tong University
No. 3 Chen Feng, Shanghai Jiao Tong University IV. mRNA Transcription Why do we need a messenger RNA? • The genetic information needs to be read “one sentence at a time”, just like we speak in sentences. Genes are “sentences” in genetic information • Protein synthesis takes place away from where chromosome exists
上泽充通大学 Shanghai Jiao Tong University 7.7 Overview of transcription 7.7.1 RNA polymerase RNA polymerase requires DNA as a template Direction:5'→3'. Do not need primer:RNA polymerase can start chains Triphosphate-containing:The initial nucleotide in an RNA retains 3 phosphate The first base in the rNa is always a purine. No.4 Chen Feng.Shanghai Jiao Tong University
No. 4 Chen Feng, Shanghai Jiao Tong University 7.7 Overview of transcription RNA polymerase requires DNA as a template Direction: 5’ 3’. Do not need primer: RNA polymerase can start chains Triphosphate-containing: The initial nucleotide in an RNA retains 3 phosphate The first base in the RNA is always a purine. 7.7.1 RNA polymerase
上泽克通大学 Shanghai Jiao Tong University Overview of Transcription Transcription(DNA to RNA)is carried out by RNA polymerase RNA polymerase uses DNA as template RNA precursors are ATP,GTP,CTP,and UTP Chain growth is 5'to 3'just like DNA replication No.5 2012 Pearson Education,Inc. Chen Feng.Shanghai Jiao Tong University
No. 5 Chen Feng, Shanghai Jiao Tong University Overview of Transcription Transcription (DNA to RNA) is carried out by RNA polymerase • RNA polymerase uses DNA as template • RNA precursors are ATP, GTP, CTP, and UTP • Chain growth is 5′ to 3′ just like DNA replication © 2012 Pearson Education, Inc