DNA fingerprinting? What is a nucleosome?What role do histones play in nucleosomes? How do chromatin modifications regulate transcription?What modifications are observed in regions of the genome that are actively transcribed and not actively transcribed？ How would a chromosome be affected if it lacked (a)replication origins or(b)a centromere? Can proteins be synthesized inside mitochondria?Are all the mitochondrial proteins synthesized within mitochondria? 七、学习资源、课外自主学习参考 (可以列出供学生进一步学习、拓展本章节内容的网站、著作、期刊的名称及内容等) Molecular Biology of the Cell,Alberts等编著，第六版，2014年出版，Garland Science Molecular Biology:Principles and Practice,Michael Cox等编著，第二版，2015 年出版，W.H.Freeman and Company Molecular Biology,Robert Weaver,,第五版，2012年出版，McGraw-Hil
DNA fingerprinting? • What is a nucleosome? What role do histones play in nucleosomes? • How do chromatin modifications regulate transcription? What modifications are observed in regions of the genome that are actively transcribed and not actively transcribed? • How would a chromosome be affected if it lacked (a) replication origins or (b) a centromere? • Can proteins be synthesized inside mitochondria? Are all the mitochondrial proteins synthesized within mitochondria? 七、学习资源、课外自主学习参考 (可以列出供学生进一步学习、拓展本章节内容的网站、著作、期刊的名称及内容等) Molecular Biology of the Cell，Alberts 等编著，第六版，2014 年出版，Garland Science Molecular Biology: Principles and Practice, Michael Cox 等编著, 第二版，2015 年出版，W. H. Freeman and Company Molecular Biology, Robert Weaver, 第五版，2012 年出版，McGraw-Hill
苏州大学医学部药学院授课教案 授课教师：滕昕辰 授课日期：2021年3月30日 课程名称 Molecular cell biology(英文) 所属学科 药学 Molecular Cell Biology,Lodish、Berk等编 本科三年级第 教材名称 授课年级 著，第八版 一学期 授课章节 5.Control of gene expression 授课时间 150分钟 一、教学目标：含知识目标、技能（能力）目标 1.知识目标： (1)Understand the mechanism of gene transcription regulation in prokaryotes (2)Understand the mechanism of gene transcription regulation in eukaryotes (3)Understand the mechanism of post-transcriptional gene control in eukaryotes 2.技能（能）目标： (1)Learn basic English terms in molecular biology (2)Improve the skills in listening and speaking of English in molecular biology 二、授课内容（依据教学大纲）及时间分配 Lecture 1: (1)Transcription initiation by bacterial RNA polymerase(10 min) (2)Regulation of transcription initiation of lac operon(15 min) (3)Regulation of transcription initiation of Trp operon(10 min) (4)Regulation of transcription elongation and termination of Trp operon(10 min) (5)Riboswitch controls transcription termination(5 min) Lecture 2 (6)Regulatory elements in eukaryotic DNA(5 min) (7)Function of general transcription factors(5 min) (8)Structure of specific transcription factors(10 min)
苏州大学医学部药学院授课教案 授课教师：滕昕辰 授课日期：2021 年 3 月 30 日 课程名称 Molecular cell biology（英文） 所属学科 药学 教材名称 Molecular Cell Biology，Lodish、Berk 等编 著，第八版 授课年级 本科三年级第 一学期 授课章节 5. Control of gene expression 授课时间 150 分钟 一、教学目标：含知识目标、技能（能力）目标 1．知识目标： (1) Understand the mechanism of gene transcription regulation in prokaryotes (2) Understand the mechanism of gene transcription regulation in eukaryotes (3) Understand the mechanism of post-transcriptional gene control in eukaryotes 2. 技能（能力）目标： (1) Learn basic English terms in molecular biology (2) Improve the skills in listening and speaking of English in molecular biology 二、授课内容（依据教学大纲）及时间分配 Lecture 1: (1) Transcription initiation by bacterial RNA polymerase (10 min) (2) Regulation of transcription initiation of lac operon (15 min) (3) Regulation of transcription initiation of Trp operon (10 min) (4) Regulation of transcription elongation and termination of Trp operon (10 min) (5) Riboswitch controls transcription termination (5 min) Lecture 2 (6) Regulatory elements in eukaryotic DNA (5 min) (7) Function of general transcription factors (5 min) (8) Structure of specific transcription factors (10 min)
(9)Transcription factor interactions increase gene-control options(5 min) (10)The mediator complex(5 min) (11)Molecular mechanisms of transcription repression and activation(10 min) (12)Regulation of transcription factor(5 min) (13)Transcription Initiation by Pol I and Pol III(5 min) Lecture 3: (14)Regulation of pre-mRNA processing (alternative splicing)(15 min) (15)Transport of mRNA across the nuclear envelope(7 min) (16)Degradation of mRNAs in the cytoplasm occurs by several mechanisms(5 min) (17)RNA interference induces Degradation of mRNA(10 min) (18)Cytoplasmic polyadenylation promotes translation of some mRNAs(5 min) (19)Protein synthesis can be globally regulated(TORC1,eIF2 kinases)(8 min) 三、教学重点与难点 1.教学重点 (1)Sigma factor (2)lac operon,trp operon (3)Multiple transcription-control elements (4)Structure,functions and regulation of transcription factors (5)Post-transcriptional regulation of gene expression 2.教学难点 (1)Two-component regulatory systems in bacteria (2)Molecular mechanism of transcription repression and activation (3)Function of enhanceosome and mediator (4)RNA interference 四、教学方法选择
(9) Transcription factor interactions increase gene-control options (5 min) (10) The mediator complex (5 min) (11) Molecular mechanisms of transcription repression and activation (10 min) (12) Regulation of transcription factor (5 min) (13) Transcription Initiation by Pol I and Pol III (5 min) Lecture 3: (14) Regulation of pre-mRNA processing (alternative splicing) (15 min) (15) Transport of mRNA across the nuclear envelope (7 min) (16) Degradation of mRNAs in the cytoplasm occurs by several mechanisms (5 min) (17) RNA interference induces Degradation of mRNA (10 min) (18) Cytoplasmic polyadenylation promotes translation of some mRNAs (5 min) (19) Protein synthesis can be globally regulated (TORC1, eIF2 kinases) (8 min) 三、教学重点与难点 1．教学重点 (1) Sigma factor (2) lac operon, trp operon (3) Multiple transcription-control elements (4) Structure, functions and regulation of transcription factors (5) Post-transcriptional regulation of gene expression 2. 教学难点 (1) Two-component regulatory systems in bacteria (2) Molecular mechanism of transcription repression and activation (3) Function of enhanceosome and mediator (4) RNA interference 四、教学方法选择
1.理论课堂教学 A.多媒体教学：（简要列出本章节多媒体教学设计特点及优势） (1)Illustration of structure and function of sigma factors (2)Illustration of mechanism of operon regulation in bacteria (3)Illustration of regulatory elements in eukaryotic cells (4)Illustration of mechanism of transcription regulation in eukaryotic cells (5)Illustration of mechanism of post-transcriptional regulation in eukaryotic cells B.互动教学：（根据实际情况列出案例/实例讨论、分析讨论、分组讨论、翻转课堂 等互动教学内容标题) (1)Why do single-celled prokaryotes control gene expression at specific times? (2)What happens if there is no mediator complex? (3)Can alternative splicing of mRNA explain the differences between different tissues and cells? (4)Is there directivity in the nucleation of mRNA?How to prove this experimentally? (5)Why do the same genomes produce different proteomes? (6)Why can a single fertilized egg cell become a variety of cell types? (7)Can you predict the application of RNA interference 2.实验课教学 Not applicable. 五、更新或补充内容提要 课后总结：按照实际进度对知识点分详略进行讲解 Emphasize the key components of gene expression regulation 六、复习思考题 Describe the molecular events that occur at the lac operon when E.coli cells are shifted from a glucose-containing medium to a lactose-containing medium. Describe three types of post-transcriptional regulation of protein-coding genes. Why there are about 20000 genes in human but only ~1400 transcriptional factors?
1. 理论课堂教学 A. 多媒体教学：（简要列出本章节多媒体教学设计特点及优势） (1) Illustration of structure and function of sigma factors (2) Illustration of mechanism of operon regulation in bacteria (3) Illustration of regulatory elements in eukaryotic cells (4) Illustration of mechanism of transcription regulation in eukaryotic cells (5) Illustration of mechanism of post-transcriptional regulation in eukaryotic cells B. 互动教学：（根据实际情况列出案例/实例讨论、分析讨论、分组讨论、翻转课堂 等互动教学内容标题） (1) Why do single-celled prokaryotes control gene expression at specific times? (2) What happens if there is no mediator complex? (3) Can alternative splicing of mRNA explain the differences between different tissues and cells? (4) Is there directivity in the nucleation of mRNA? How to prove this experimentally? (5) Why do the same genomes produce different proteomes? (6) Why can a single fertilized egg cell become a variety of cell types? (7) Can you predict the application of RNA interference 2. 实验课教学 Not applicable. 五、更新或补充内容提要 课后总结：按照实际进度对知识点分详略进行讲解 Emphasize the key components of gene expression regulation 六、复习思考题 • Describe the molecular events that occur at the lac operon when E. coli cells are shifted from a glucose-containing medium to a lactose-containing medium. • Describe three types of post-transcriptional regulation of protein-coding genes. • Why there are about 20000 genes in human but only ~1400 transcriptional factors?
Describe the structural features and function of transcription activator and repressor proteins. How can transcription factors binding onto enhancers thousands of base pairs from the transcription start site affect the transcription initiation? How are transcriptional factors regulated by extracellular signals? Why there are much more proteins than genes in eukaryotic cells? What are the differences between miRNAs and siRNAs? List all the reasons you can think of to explain decreased expression of a protein. 七、学习资源、课外自主学习参考 (可以列出供学生进一步学习、拓展本章节内容的网站、著作、期刊的名称及内容等) Molecular Biology of the Cell,Alberts等编著，第六版，2014年出版，Garland Science Molecular Biology::Principles and Practice,,Michael Cox等s编著，第二版，20l5 年出版，W.H.Freeman and Company Molecular Biology,Robert Weaver,第五版，2012年出版，McGraw-Hil 苏州大学医学部药学院授课教案 授课教师：滕昕辰 授课日期：2021年4月6日
• Describe the structural features and function of transcription activator and repressor proteins. • How can transcription factors binding onto enhancers thousands of base pairs from the transcription start site affect the transcription initiation? • How are transcriptional factors regulated by extracellular signals? • Why there are much more proteins than genes in eukaryotic cells? • What are the differences between miRNAs and siRNAs? • List all the reasons you can think of to explain decreased expression of a protein. 七、学习资源、课外自主学习参考 (可以列出供学生进一步学习、拓展本章节内容的网站、著作、期刊的名称及内容等) Molecular Biology of the Cell，Alberts 等编著，第六版，2014 年出版，Garland Science Molecular Biology: Principles and Practice, Michael Cox 等编著, 第二版，2015 年出版，W. H. Freeman and Company Molecular Biology, Robert Weaver, 第五版，2012 年出版，McGraw-Hill 苏州大学医学部药学院授课教案 授课教师：滕昕辰 授课日期：2021 年 4 月 6 日