苏州大学医学部 教 案 课程名称： Biochemistry V Experiment 授课对象： 2019级药学 授课学时： 36 Experimental Biochemistry Essentials 教材版本： 生物化学与分子生物学实验教程徐 岚、钱晖科学出版社，2014 主讲教师： 刘晶晶/高媛 苏州大学基础医学与生物科学学院制 2020年09月20日
苏州大学医学部 教 案 课程名称： Biochemistry V Experiment 授课对象： 2019 级药学 授课学时： 36 教材版本： Experimental Biochemistry Essentials 生物化学与分子生物学实验教程 徐 岚、钱晖 科学出版社，2014 主讲教师： 刘晶晶/高媛 苏州大学基础医学与生物科学学院制 2020 年 09 月 20 日
Teaching plan of Soochow University Subjects:Biochemistry Experiment Class:2019 Pharmaceutical sciences Date:2020.10. 23/Fri/5-8 Chapter Text page:N/A Teaching duration:200 Title Introduction to basic techniques of biochemistry experiment 1.Understand the principle of centrifugation and know how to manipulate centrifuge Teaching goal 2.Understand Beer-Lambert Law and the method to measure substance concentration using spectrophotometer 3.Learn to use pipets 1、 Content: (1)入Lab safty training实10 (2)Lab report writing requirement 10 Teaching content、 (3)Use glass pipet 10 (4)Principle of centrifugation,types of centrifuge and how to use centrifuge,lab Teaching nethod、 practice I 30' (5),Principle of spectrophotometer,learn to use spectrophotometer,lab practice 2 Teaching emphasis、 30 difficulty、 (6)Student practices 110' time distribution 2.Key point:Learn to use centrifuge and spectrophotometer. Teaching method:Teacher should explain and display the experiment according to powerpoint Teaching Powerpoint,glass pipet,centrifuge,spectrophotometer instrument
Teaching plan of Soochow University Subjects：Biochemistry Experiment Class：2019 Pharmaceutical sciences Date：2020. 10. 23/Fri/5-8 Chapter 1 Text page：N/A Teaching duration: 200 Title Introduction to basic techniques of biochemistry experiment Teaching goal 1.Understand the principle of centrifugation and know how to manipulate centrifuge 2.Understand Beer-Lambert Law and the method to measure substance concentration using spectrophotometer 3. Learn to use pipets Teaching content、 Teaching method、 Teaching emphasis、 difficulty、 time distribution 1、 Content： （1）、Lab safty training 实 10‘ （2）、Lab report writing requirement 10‘ （3）Use glass pipet 10‘ （4）、Principle of centrifugation, types of centrifuge and how to use centrifuge，lab practice I 30’ （5）、Principle of spectrophotometer, learn to use spectrophotometer, lab practice 2 30’ （6）、Student practices 110’ 2、 Key point：Learn to use centrifuge and spectrophotometer。 Teaching method：Teacher should explain and display the experiment according to powerpoint . Teaching instrument Powerpoint,glass pipet, centrifuge, spectrophotometer
1. Will look down or look up the scale affect the volume during pipetting Question and quiz in 2.In room temperature,is the absorption proportional to the concentration of the class solution? After class 1.Emphasize the importance to balance the sample before centrifugation summary 2.Emphasize the use proper blank to calibrate spectrophotometer
Question and quiz in class 1. Will look down or look up the scale affect the volume during pipetting 2. In room temperature, is the absorption proportional to the concentration of the solution? After class summary 1. Emphasize the importance to balance the sample before centrifugation 2. Emphasize the use proper blank to calibrate spectrophotometer
Teaching plan of Soochow University Subjects:Biochemistry Experiment Class:2019 Pharmaceutical sciences Date:2020.10 30/Fri/5-8 Chapter 3 Text page:N/A Teaching duration:200 Title Effects of some factors on enzymatic activity (a-salivary amylase) Teaching 1.Understand the properties of enzyme goal 2.Understand how factors such as temperature and pH affect enzymatic activity. l、Content: 1.Review mechanism of action of enzymes 10' 2.Review how temperature affect the Km and reaction speed 5' 3.Review how pH affect the Km of enzyme 5' Teaching 4. Review types of inhibitors,and how inhibitor affect the Km and Vmax of content、 enzyme 10 Teaching 5.How to design assays to test enzymatic activity.5' method、 6.Activity of amylase and assay to test amylase activity 5' Teaching 7.Experimental procedures 10' emphasis、 difficulty、 8.Students'experiment 150' time distribution 2、Key point: make sure saliva should be added the same time for all experimental groups 3.Teaching method:Teacher should explain and display the experiment according to powerpoint. Teaching Powerpoint,,glass pipet, instrument
Teaching plan of Soochow University Subjects：Biochemistry Experiment Class：2019 Pharmaceutical sciences Date：2020. 10. 30/Fri/5-8 Chapter 3 Text page：N/A Teaching duration: 200 Title Effects of some factors on enzymatic activity (a-salivary amylase) Teaching goal 1.Understand the properties of enzyme 2.Understand how factors such as temperature and pH affect enzymatic activity. Teaching content、 Teaching method、 Teaching emphasis、 difficulty、 time distribution 1、 Content： 1. Review mechanism of action of enzymes 10’ 2. Review how temperature affect the Km and reaction speed 5’ 3. Review how pH affect the Km of enzyme 5’ 4. Review types of inhibitors, and how inhibitor affect the Km and Vmax of enzyme 10’ 5. How to design assays to test enzymatic activity. 5’ 6. Activity of amylase and assay to test amylase activity 5’ 7. Experimental procedures 10’ 8. Students’ experiment 150’ 2、 Key point： make sure saliva should be added the same time for all experimental groups 3、Teaching method： Teacher should explain and display the experiment according to powerpoint . Teaching instrument Powerpoint,,glass pipet
Question 1.Name the types of inhibitors according to the mechanism of action? and quiz in class 2.How temperature/pH affect enzyme activity? After class When testing the effect of low temperature,make sure to put the tubes on ice summary immediately after adding saliva
Question and quiz in class 1. Name the types of inhibitors according to the mechanism of action? 2. How temperature/pH affect enzyme activity? After class summary When testing the effect of low temperature, make sure to put the tubes on ice immediately after adding saliva