1.理论课堂教学 A.多媒体教学：（简要列出本章节多媒体教学设计特点及优势） (1)Illustration of RNA polymerase structure (2)Illustration of three stages in transcription:initiation,elongation,and termination (3)Illustration of eukaryotic pre-mRNA processing (4)Illustration of decoding of mRNA by tRNAs (5)Illustration of stepwise synthesis of proteins on ribosomes B.互动教学：（根据实际情况列出案例/实例讨论、分析讨论、分组讨论、翻转课堂 等互动教学内容标题) (1)Is the proofreading function of RNA polymerase different from that of DNA polymerase? (2)Why does RNA transcription tolerate a high error rate? (3)Why is mRNA more stable when it is added a 5 'cap or a 3'poly-A tail? (4)There are 61 codons,but how can many cells have less than 61 tRNAs? (5)What is the proofreading mechanism of protein translation? 2.实验课教学 Not applicable. 五、更新或补充内容提要 课后总结：按照实际进度对知识点分详略进行讲解 Emphasize the key components of transcription and translation 六、复习思考题 What are the differences of central dogma between prokaryotes and eukaryotes? Can you write the mRNA sequence (or non-template DNA sequence)when given the template DNA strand sequence? What is the proofreading mechanism in RNA synthesis? How is eukaryotic pre-mRNA processed?
1. 理论课堂教学 A. 多媒体教学：（简要列出本章节多媒体教学设计特点及优势） (1) Illustration of RNA polymerase structure (2) Illustration of three stages in transcription: initiation, elongation, and termination (3) Illustration of eukaryotic pre-mRNA processing (4) Illustration of decoding of mRNA by tRNAs (5) Illustration of stepwise synthesis of proteins on ribosomes B. 互动教学：（根据实际情况列出案例/实例讨论、分析讨论、分组讨论、翻转课堂 等互动教学内容标题） (1) Is the proofreading function of RNA polymerase different from that of DNA polymerase? (2) Why does RNA transcription tolerate a high error rate? (3) Why is mRNA more stable when it is added a 5 'cap or a 3' poly-A tail? (4) There are 61 codons, but how can many cells have less than 61 tRNAs? (5) What is the proofreading mechanism of protein translation? 2. 实验课教学 Not applicable. 五、更新或补充内容提要 课后总结：按照实际进度对知识点分详略进行讲解 Emphasize the key components of transcription and translation 六、复习思考题 • What are the differences of central dogma between prokaryotes and eukaryotes? • Can you write the mRNA sequence (or non-template DNA sequence) when given the template DNA strand sequence? • What is the proofreading mechanism in RNA synthesis? • How is eukaryotic pre-mRNA processed?
What differences can you identify between DNA synthesis and RNA synthesis? What are the proofreading mechanism in protein synthesis? ● What are the functions of A,P,E sites in ribosomes during translation? What are the functions of GTP-biding proteins in translation? 七、学习资源、课外自主学习参考 (可以列出供学生进一步学习、拓展本章节内容的网站、著作、期刊的名称及内容等) Molecular Biology of the Cell,Alberts等编著，第六版，2014年出版，Garland Science Molecular Biology:Principles and Practice,Michael Cox等编著，第二版，2015 年出版，W.H.Freeman and Company Molecular Biology,Robert Weaver,第五版，20l2年出版，McGraw-Hil 苏州大学医学部药学院授课教案
• What differences can you identify between DNA synthesis and RNA synthesis? • What are the proofreading mechanism in protein synthesis? • What are the functions of A, P, E sites in ribosomes during translation? • What are the functions of GTP-biding proteins in translation? 七、学习资源、课外自主学习参考 (可以列出供学生进一步学习、拓展本章节内容的网站、著作、期刊的名称及内容等) Molecular Biology of the Cell，Alberts 等编著，第六版，2014 年出版，Garland Science Molecular Biology: Principles and Practice, Michael Cox 等编著, 第二版，2015 年出版，W. H. Freeman and Company Molecular Biology, Robert Weaver, 第五版，2012 年出版，McGraw-Hill 苏州大学医学部药学院授课教案
授课教师：滕昕辰 授课日期：2021年3月23日 课程名称 Molecular cell biology(英文) 所属学科 药学 Molecular Cell Biology,Lodish、Berk等编 本科三年级第 教材名称 授课年级 著，第八版 一学期 授课章节 4.Genes,genomics and chromosomes 授课时间 150分钟 一、教学目标：含知识目标、技能（能力)目标 1.知识目标： (1)Understand the structure of genes and all the functional elements within a gene (2)Be able to identify single genes,gene families,and pseudogenes (3)Be able to understand the influence of various non-coding DNA on genome formation (4)Understand how DNA is folded step by step to form the higher structure of chromosomes (5)Understand the effect of histone tail modification on chromosome structure and transcription 2.技能（能力）目标： (1)Learn basic English terms in molecular biology (2)Improve the skills in listening and speaking of English in molecular biology 二、授课内容（依据教学大纲）及时间分配 Lecture 1: (1)Molecular definition of a gene(5 min) (2)Structure of genes in prokaryotic cells(15 min) (3)Structure of genes in eukaryotic cells(15 min) (4)Organization of genes on chromosomes(5 min) (5)Protein-coding genes(10 min) Lecture 2: (6)Non-protein coding genes encode functional RNAs(5 min)
授课教师：滕昕辰 授课日期：2021 年 3 月 23 日 课程名称 Molecular cell biology（英文） 所属学科 药学 教材名称 Molecular Cell Biology，Lodish、Berk 等编 著，第八版 授课年级 本科三年级第 一学期 授课章节 4. Genes, genomics and chromosomes 授课时间 150 分钟 一、教学目标：含知识目标、技能（能力）目标 1．知识目标： (1) Understand the structure of genes and all the functional elements within a gene (2) Be able to identify single genes, gene families, and pseudogenes (3) Be able to understand the influence of various non-coding DNA on genome formation (4) Understand how DNA is folded step by step to form the higher structure of chromosomes (5) Understand the effect of histone tail modification on chromosome structure and transcription 2. 技能（能力）目标： (1) Learn basic English terms in molecular biology (2) Improve the skills in listening and speaking of English in molecular biology 二、授课内容（依据教学大纲）及时间分配 Lecture 1: (1) Molecular definition of a gene (5 min) (2) Structure of genes in prokaryotic cells (15 min) (3) Structure of genes in eukaryotic cells (15 min) (4) Organization of genes on chromosomes (5 min) (5) Protein-coding genes (10 min) Lecture 2: (6) Non-protein coding genes encode functional RNAs (5 min)
(7)Simple-sequence DNAs:Microsatellites(15 min) (8)Interspersed repeats:DNA Transposons,retrotransposons(LTRs,Non-LTRs (LINEs,SINEs))(30 min) Lecture 3: (9)Chromatin exists in extended and condensed forms(20 min) (10)Modification of histone tails control chromatin condensation and function(10 min) (11)Functional elements of eukaryotic chromosomes:replication origins, centromere,telomere(10 min) (12)Structure of mitochondrial genome(10 min) 三、教学重点与难点 1.教学重点 (1)Structure of genes and all the functional elements within a gene (2)Structure of chromosomes (3)Differences of gene structure and chromosome structure between prokaryotic and eukaryotic cells (4)Composition of human genome 2.教学难点 (1)Differences of gene structure between prokaryotic and eukaryotic cells (2)Modification of histone and structure of chromosome (3)Non-coding DNAs in human genome 四、教学方法选择
(7) Simple-sequence DNAs: Microsatellites (15 min) (8) Interspersed repeats: DNA Transposons, retrotransposons (LTRs, Non-LTRs (LINEs, SINEs)) (30 min) Lecture 3: (9) Chromatin exists in extended and condensed forms (20 min) (10) Modification of histone tails control chromatin condensation and function (10 min) (11) Functional elements of eukaryotic chromosomes: replication origins, centromere, telomere (10 min) (12) Structure of mitochondrial genome (10 min) 三、教学重点与难点 1．教学重点 (1) Structure of genes and all the functional elements within a gene (2) Structure of chromosomes (3) Differences of gene structure and chromosome structure between prokaryotic and eukaryotic cells (4) Composition of human genome 2. 教学难点 (1) Differences of gene structure between prokaryotic and eukaryotic cells (2) Modification of histone and structure of chromosome (3) Non-coding DNAs in human genome 四、教学方法选择
1.理论课堂教学 A.多媒体教学：（简要列出本章节多媒体教学设计特点及优势） (1)Illustrations of differences between the prokaryotic and eukaryotic cells (2)Illustrations of structure of genes and chromosomes in prokaryotic and eukaryotic cells (3)Illustration of mechanism of transposons (4)Illustration of structure of chromosome B.互动教学：（根据实际情况列出案例/实例讨论、分析讨论、分组讨论、翻转课堂 等互动教学内容标题) (1)A prokaryotic operon can be transcribed into how many transcription units,how many proteins? (2)What is the physiological significance of complex transcription units in eukaryotes? (3)What kind of genes need multiple copies? (4)Can transposons cause severe mutations? (5)Between the loose and compact chromosome,which one is easier to be transcribed? 2.实验课教学 Not applicable. 五、更新或补充内容提要 课后总结：按照实际进度对知识点分详略进行讲解 Emphasize the key components of genome and chromosomes 六、复习思考题 What are the differences of gene structure between eukaryotes and prokaryotes? Can you give a big picture of the human genome compositions? Describe the differences between solitary genes,gene families,pseudogenes, and tandemly repeated genes. How is the repetitious DNA useful for identifying individuals by the technique of
1. 理论课堂教学 A. 多媒体教学：（简要列出本章节多媒体教学设计特点及优势） (1) Illustrations of differences between the prokaryotic and eukaryotic cells (2) Illustrations of structure of genes and chromosomes in prokaryotic and eukaryotic cells (3) Illustration of mechanism of transposons (4) Illustration of structure of chromosome B. 互动教学：（根据实际情况列出案例/实例讨论、分析讨论、分组讨论、翻转课堂 等互动教学内容标题） (1) A prokaryotic operon can be transcribed into how many transcription units, how many proteins? (2) What is the physiological significance of complex transcription units in eukaryotes? (3) What kind of genes need multiple copies? (4) Can transposons cause severe mutations? (5) Between the loose and compact chromosome, which one is easier to be transcribed? 2. 实验课教学 Not applicable. 五、更新或补充内容提要 课后总结：按照实际进度对知识点分详略进行讲解 Emphasize the key components of genome and chromosomes 六、复习思考题 • What are the differences of gene structure between eukaryotes and prokaryotes? • Can you give a big picture of the human genome compositions? • Describe the differences between solitary genes, gene families, pseudogenes, and tandemly repeated genes. • How is the repetitious DNA useful for identifying individuals by the technique of